切换至 "中华医学电子期刊资源库"
高级检索
中华结直肠疾病电子杂志

中华结直肠疾病电子杂志 ›› 2015, Vol. 04 ›› Issue (04) : 396 -400. doi: 10.3877/cma.j.issn.2095-3224.2015.04.10

所属专题： 文献；

论著

MACC1在结直肠癌迁移及化疗耐药中的实验研究
王刚1, 鞠海星1, 付志璇1, 李德川,1   
  1. 1. 310022　杭州，浙江省肿瘤医院结直肠外科
  • 收稿日期:2015-05-12 出版日期:2015-08-25
  • 通信作者: 李德川
  • 基金资助:
    浙江省自然科学基金(Y207427)

The role of MACC1 in the migration and chemoresistance of colorectal cancer

Gang WANG1, Hai-xing JU1, Zhi-xuan FU1, De-chuan LI,1   

  1. 1. Department of colorectal cancer surgery, Zhe jiang cancer hospital, Hangzhou 310012, China
  • Received:2015-05-12 Published:2015-08-25
  • Corresponding author: De-chuan LI
  • About author:
    Corresponding author: LI De-chuan, Email:
全文 ( 0 ) 下载PDF ( 2 ) 导出引用
引用本文：

王刚, 鞠海星, 付志璇, 李德川. MACC1在结直肠癌迁移及化疗耐药中的实验研究[J/OL]. 中华结直肠疾病电子杂志, 2015, 04(04): 396-400.

Gang WANG, Hai-xing JU, Zhi-xuan FU, De-chuan LI. The role of MACC1 in the migration and chemoresistance of colorectal cancer[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2015, 04(04): 396-400.

目的

探讨MACC1在结直肠癌迁移及化疗耐药中的作用。

方法

采用SiRNA干扰技术沉默MACC1表达，利用Transwell迁移实验研究MACC1在结直肠癌迁移中的作用，应用MTT法研究MACC1在结直肠癌化疗耐药中的作用。Western Blot检测相关通路蛋白表达变化。

结果

SW480细胞系MACC1表达极低，而SW620细胞系中MACC1蛋白呈高表达状态。SiRNA技术可显著抑制MACC1的表达水平。MACC1干扰后，SW620细胞发生迁移的数目显著减少(P<0.05)且对5-Fu处理表现更为敏感(P<0.05)。下调MACC1表达后提示p-ERK及p-Met蛋白表达水平随之显著减少。

结论

MACC1在结直肠癌转移及耐药过程中发挥重要作用，并有可能成为潜在治疗靶点。

关键词: 结直肠肿瘤, 细胞迁移分析, 抗药性，肿瘤
Objective

To investigate the functions of metastasis-associated in colon cancer-1(MACC1)in the migration and chemoresistance of colorectal cancer.

Methods

SiRNA was used to inhibit the expression of MACC1.Transwell assay was performed to study the role of MACC1 in migration.MTT assay was used to detect the chemosensitivity of SW620 cells to 5-Fluorouracil.The relevant protein changes of potential signaling pathways were determined by Western Blot.

Results

Compared with SW480 cells, SW620 cells showed high expression of MACC1, which could be inhibited by SiRNA transfection.Inhibition of MACC1 by SiRNA significantly suppressed colorectal cancer cell migration.We found that down-regulation of MACC1 sensitized SW620 cells to 5-Fu treatment.Furthermore, the levels of p-ERK and p-Met were also suppressed by inhibition of MACC1.

Conclusion

Our findings suggest that MACC1 plays an important role in the migration and chemoresistance of colorectal cancer, and may serve as a potential therapeutic target.

Key words: Colorectal neoplasms, Cell migration assays, Drug resistance, neoplasm
图1 MACC1在不同细胞系中表达情况及干扰验证实验图
图2 干扰MACC1表达后，SW620细胞迁移能力的改变实验图像
图3 干扰MACC1表达后，SW620细胞用5-Fu处理后受抑制情况曲线图
图4 干扰MACC1表达后，相关通路蛋白的表达变化实验图
[1]
Stein U, Walther W, Arlt F, et al.MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis.Nat Med, 2009, 15(1): 59-67.
[2]
Zhen T, Dai S, Li H, et al.MACC1 promotes carcinogenesis of colorectal cancer via beta-catenin signaling pathway.Oncotarget, 2014, 5(11): 3756-3769.
[3]
Isella C, Mellano A, Galimi F, et al.MACC1 mRNA levels predict cancer recurrence after resection of colorectal cancer liver metastases.Ann Surg, 2013, 257(6): 1089-1095.
[4]
Tokarz P, Blasiak J. The role of microRNA in metastatic colorectal cancer and its significance in cancer prognosis and treatment.Acta Biochim Pol, 2012, 59(4): 467-474.
[5]
Wang G, Kang MX, Lu WJ, et al.MACC1: A potential molecule associated with pancreatic cancer metastasis and chemoresistance.Oncol Lett, 2012, 4(4): 783-791.
[6]
Guo T, Yang J, Yao J, et al.Expression of MACC1 and c-Met in human gastric cancer and its clinical significance.Cancer Cell Int, 2013, 13(1): 121.
[7]
Lin L, Huang H, Liao W, et al.MACC1 supports human gastric cancer growth under metabolic stress by enhancing the Warburg effect.Oncogene, 2014.
[8]
Ji D, Lu ZT, Li YQ, et al.MACC1 expression correlates with PFKFB2 and survival in hepatocellular carcinoma.Asian Pac J Cancer Prev, 2014, 15(2): 999-1003.
[9]
Qiu J, Huang P, Liu Q, et al.Identification of MACC1 as a novel prognostic marker in hepatocellular carcinoma.J Transl Med, 2011, 9: 166.
[10]
Shirahata A, Sakata M, Kitamura Y, et al.MACC 1 as a marker for peritoneal-disseminated gastric carcinoma.Anticancer Res, 2010, 30(9): 3441-3444.
[11]
Wang G, Fu Z, Li D. MACC1 overexpression and survival in solid tumors: a meta-analysis.Tumour Biol, 2014.
[12]
Fang JY, Richardson BC.The MAPK signalling pathways and colorectal cancer.Lancet Oncol, 2005, 6(5): 322-327.
[13]
Perez-Vargas JC, Biondani P, Maggi C, et al.Role of cMET in the development and progression of colorectal cancer.Int J Mol Sci, 2013, 14(9): 18056-18077.
[14]
Kammula US, Kuntz EJ, Francone TD, et al.Molecular co-expression of the c-Met oncogene and hepatocyte growth factor in primary colon cancer predicts tumor stage and clinical outcome.Cancer Lett, 2007, 248(2): 219-228.
[15]
Di Renzo MF, Olivero M, Giacomini A, et al.Overexpression and amplification of the met/HGF receptor gene during the progression of colorectal cancer.Clin Cancer Res, 1995, 1(2): 147-154.
[1] 罗青杉, 梅海涛, 郝家领, 蔡锦锋, 周润楷, 温玉刚. 连接蛋白43通过调控细胞周期抑制结直肠癌的增殖机制研究[J/OL]. 中华普通外科学文献(电子版), 2024, 18(05): 344-349.
[2] 徐逸男. 不同术式治疗梗阻性左半结直肠癌的疗效观察[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 72-75.
[3] 谢丽春, 欧庆芬, 张秋萍, 叶升. 简化和标准肝脏MRI方案在结直肠癌肝转移患者随访中的临床应用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(04): 434-437.
[4] 施烨鑫, 马翔, 鲁明, 夏青城, 王鹏超, 宋青雨, 赵庆洪. 腹腔镜下结直肠肿瘤定位研究进展[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(04): 463-466.
[5] 韩加刚, 王振军. 梗阻性左半结肠癌的治疗策略[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 450-458.
[6] 梁轩豪, 李小荣, 李亮, 林昌伟. 肠梗阻支架置入术联合新辅助化疗治疗结直肠癌急性肠梗阻的疗效及其预后的Meta 分析[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 472-482.
[7] 严虹霞, 王晓娟, 张毅勋. 2 型糖尿病对结直肠癌患者肿瘤标记物、临床病理及预后的影响[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 483-487.
[8] 赵磊, 刘文志, 林峰, 于剑, 孙铭骏, 崔佑刚, 张旭, 衣宇鹏, 于宝胜, 冯宁. 深部热疗在改善结直肠癌术后辅助化疗副反应及生活质量中的作用研究[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 488-493.
[9] 黄海洋, 邝永龙, 陈嘉胜. 基层医院结直肠肿瘤经自然腔道取标本手术30 例分析[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 510-518.
[10] 任佳琪, 刁德昌, 何自衍, 张雪阳, 唐新, 李文娟, 李洪明, 卢新泉, 易小江. 网膜融合线导向的脾曲游离技术在左半结肠癌根治术中的应用[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(05): 362-367.
[11] 张迪, 王春霞, 张学东, 李发馨, 庞淅文, 陈一锋, 张维胜, 王涛. 梗阻性左半结直肠癌自膨式金属支架置入后行腹腔镜手术与开腹手术的短期临床疗效比较[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(05): 375-380.
[12] 张蔚林, 王哲学, 白峻阁, 黄忠诚, 肖志刚. 利用TCGA数据库构建基于miRNA的结直肠癌列线图预后模型[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(05): 381-388.
[13] 张伟伟, 陈启, 翁和语, 黄亮. 随机森林模型预测T1 期结直肠癌淋巴结转移的初步研究[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(05): 389-393.
[14] 季鹏程, 鄂一民, 陆晨, 喻春钊. 循环外泌体相关生物标志物在结直肠癌诊断中的研究进展[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(04): 265-273.
[15] 李佳莹, 王旭丹, 梁雪, 张雷, 李佳英. 1990~2021年中国结直肠癌死亡趋势分析[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(04): 274-279.
阅读次数
全文
2
HTML PDF
最新录用 在线预览 正式出版 最新录用 在线预览 正式出版
0 0 0 0 0 2

  来源 本网站
  次数 2
  比例 100%

摘要
30
最新录用 在线预览 正式出版
0 0 30
  来源 本网站 其他网站
  次数 11 19
  比例 37% 63%

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号