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中华结直肠疾病电子杂志 ›› 2017, Vol. 06 ›› Issue (04) : 294 -300. doi: 10.3877/cma.j.issn.2095-3224.2017.04.006

所属专题: 文献

论著

MiR-196a与ZG16蛋白在结直肠癌中的表达意义及相关性分析
樊鑫鑫1, 赵青芳1, 关露露1, 刘奇1, 王茂勋1, 吕慧芳1, 陈小兵1,()   
  1. 1. 450000 郑州大学附属肿瘤医院肿瘤内科
  • 收稿日期:2017-04-07 出版日期:2017-08-25
  • 通信作者: 陈小兵
  • 基金资助:
    河南省杰出青年基金项目(No.144100510008)

Expression and their clinical significance of miR-196a and ZG16 in colorectal cancer and their correlations

Xinxin Fan1, Qingfang Zhao1, Lulu Guan1, Qi Liu1, Maoxun Wang1, Huifang Lyu1, Xiaobing Chen1,()   

  1. 1. Oncology Department, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450000, China
  • Received:2017-04-07 Published:2017-08-25
  • Corresponding author: Xiaobing Chen
  • About author:
    Corresponding author: Chen Xiaobing, Email:
引用本文:

樊鑫鑫, 赵青芳, 关露露, 刘奇, 王茂勋, 吕慧芳, 陈小兵. MiR-196a与ZG16蛋白在结直肠癌中的表达意义及相关性分析[J/OL]. 中华结直肠疾病电子杂志, 2017, 06(04): 294-300.

Xinxin Fan, Qingfang Zhao, Lulu Guan, Qi Liu, Maoxun Wang, Huifang Lyu, Xiaobing Chen. Expression and their clinical significance of miR-196a and ZG16 in colorectal cancer and their correlations[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(04): 294-300.

目的

通过测定结直肠癌患者癌组织、癌旁正常组织中miR-196a和ZG16 mRNA的相对表达水平,探讨二者与患者临床病理特征的关系及二者相关性。

方法

应用实时荧光定量PCR,检测83例结直肠癌患者癌组织、癌旁正常组织中miR-196a和ZG16 mRNA的相对表达水平,并分析二者相关性及其与患者临床病理特征之间的关系。

结果

癌组织中ZG16 mRNA表达量明显低于正常组织(t=-27.511,P<0.001);癌组织miR-196a表达量明显高于正常组织(t=9.04,P<0.001),且二者在癌组织中的表达量呈显著负相关(P<0.001,r=-0.469);癌组织中miR-196a、ZG16mRNA的表达情况与肿瘤部位、有无淋巴结转移、有无远处转移、肿瘤分期明显相关(P<0.05),与性别、年龄、肿瘤大小、分化程度、浸润深度、CEA水平无关(P>0.05)。

结论

两者可能推动结直肠癌的发生、发展,可能是潜在的病情评估及疗效评价的生物标志物;两者在左右半结直肠癌中表达量分别有差异,临床上可能有助于左右半结直肠癌靶向药物疗效预测及新的治疗靶点的发现。

Testing the expression of ZG16 mRNA and miR-196a of colorectal cancer tissue and the adjacent normal tissue to investigate the correlation between the two markers and detect their relationship with clinical pathological features of patients.

Methods

Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression level of miR-196a and ZG16 mRNA in cancer tissue and its paired adjacent normal tissue sample from 83 CRC patients. The correlation of the two markers and their relative expression level with clinical-pathological features were also analyzed.

Results

The realative expression of ZG16 mRNA was significantly lower in CRC tissues compared with adjacent normal tissue samples (t=-27.511, P<0.001); and the relative expression of miR-196a was significantly higher in cancer tissue compared with adjacent normal tissue samples (t=9.04, P<0.001), and the relative expression of ZG16 mRNA has a significantly negative correlation with the relative expression of miR-196a (P<0.001, r=-0.469); The expression of miR-196a is related to tumor location, lymph node metastasis distant metastasis and tumor stages (P<0.05), and its expression has no relationship with sex, age, the state of CEA, tumor size, differentiation degree or infiltration depthof CRC samples (P>0.05). The same results was found in the expression of ZG16 mRNA.

Conclusions

Both of miR-196a and ZG16 mRNA may promote the occurrence of colorectal cancer, and suggests that they two may be potential biomarkers for disease and curative effect evaluation. Both of the expression of the two are different in left and right CRC, which may be helpful for individual treatment.

表1 83例患者的临床病理资料
表2 主要实验试剂和仪器
图1 T1~3分别为肿瘤组织,N1~3分别为肿瘤组织对应的癌旁正常组织
表3 microRNA-196a和ZG16mRNA在癌组织和癌旁正常组织中的表达
图2 癌组织、癌旁正常组织中miR-196a和ZG16mRNA的相对表达量
表4 ZG16 mRNA与miR-196a在癌组织中相对表达量相关性分析
图3 ZG16 mRNA与miR-196a在癌组织中表达散点图
表5 miR-196a、ZG16mRNA与CRC患者临床病理特征的关系
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