通过观察骶神经电刺激（SNS）对慢传输型便秘（STC）小鼠结肠组织中5-羟色胺4受体（5-HT₄R）表达的影响，探讨SNS治疗STC的作用机制。

将30只昆明小鼠随机分为正常组和造模组，正常组10只，造模组20只。造模组采用复方地芬诺酯混悬液灌胃，正常组正常喂养。STC小鼠模型成功后，将造模组随即均分为SNS组和模型组。SNS组小鼠采用骶神经电刺激治疗；正常组与模型组于骶3神经孔对应的皮肤位置每日浅刺一次，各组均持续治疗2周。治疗结束后，检测各组小鼠粪便含水率、肠道推进率、结肠组织中5-HT₄R蛋白的表达、5-HT₄R mRNA的表达量、c-kit免疫组化表达等。

与正常组相比，模型组小鼠粪便含水率（t=22.43，P<0.05）和肠道推进率明显降低（t=12.67，P<0.05），结肠组织中5-HT₄R的蛋白表达量（t=5.96，P<0.05）、5-HT₄R mRNA的表达量（t=9.92，P<0.05）、结肠组织c-kit蛋白免疫组化的表达量（t=5.44，P<0.05）明显降低。与模型组相比，SNS组小鼠粪便含水率（t=19.36，P<0.05）和肠道推进率（t=7.47，P<0.05）明显升高，结肠组织中5-HT₄R的蛋白（t=4.76，P<0.05）及5-HT₄R mRNA的表达量（t=13.74，P<0.05）、c-kit蛋白免疫组化的表达量明显升高（t=5.39，P<0.05）。

SNS能改善STC小鼠的便秘症状，可能与上调小鼠结肠组织中5-HT₄R和c-kit的表达、促进结肠Cajal细胞的增殖及细胞功能的提升，改善肠动力有关。

To explore the mechanism of action of sacral nerve stimulation (SNS) in the treatment of slow transit constipation (STC) by observing its effect on the expression of 5-hydroxytryptamine 4 receptor (5-HT₄R) in the colonic tissue of STC mice.

Thirty Kunming mice were randomly divided into normal and modeling groups, with 10 mice in the normal group and 20 mice in the modeling group. The modeling group was gavaged with compound difenocoumarol suspension, and the normal group was fed normally. After the success of the STC mouse model, the modeling group was then divided into the SNS group and the model group. The SNS group received sacral nerve stimulation therapy; the normal group and the model group were subjected to daily acupuncture at the skin site corresponding to the S3 nerve foramen, with a 2-week treatment duration for all groups. After treatment, the fecal moisture content, intestinal propulsion rate, expression of 5-HT₄R protein, 5-HT₄R mRNA expression levels, and c-kit immunohistochemistry in the colon were measured in each group.

Compared with the normal group, the model group showed significantly reduced fecal moisture content (t=22.43, P<0.05) and intestinal propulsion rate (t=12.67, P<0.05), as well as significantly decreased expression of 5-HT₄R protein (t=5.96, P<0.05), 5-HT₄R mRNA (t=9.92, P<0.05), and c-kit protein immunohistochemistry in the colonic tissue (t=5.44, P<0.05). Compared with the model group, the SNS group exhibited significantly increased fecal moisture content (t=19.36, P<0.05) and intestinal propulsion rate (t=7.47, P<0.05), along with significantly increased expression of 5-HT₄R protein (t=4.76, P<0.05), 5-HT₄R mRNA (t=13.74, P<0.05), and c-kit protein immunohistochemistry in the colonic tissue (t=5.39, P<0.05).

Sacral nerve stimulation may alleviate constipation symptoms in STC mice, potentially associated with the upregulation of 5-HT₄R and c-kit expression in colonic tissues, which may promote the proliferation of interstitial cells of Cajal, enhance their cellular function, and ultimately improve intestinal motility.