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中华结直肠疾病电子杂志 ›› 2017, Vol. 06 ›› Issue (05) : 387 -391. doi: 10.3877/cma.j.issn.2095-3224.2017.05.007

所属专题: 文献

论著

结直肠癌组织中CyPJ的表达及其临床意义
张红波1, 陈剑2, 胡金晨1, 王熙勋1, 贾荣保1, 姜立新1,()   
  1. 1. 264000 烟台市,青岛大学附属烟台毓璜顶医院胃肠外一科
    2. 264000 烟台市,青岛大学附属烟台毓璜顶医院中心实验室
  • 收稿日期:2017-02-27 出版日期:2017-10-25
  • 通信作者: 姜立新
  • 基金资助:
    山东省优秀中青年科学家科研奖励基金(No.2008BSA06001)

Expression and clinical significance of CyPJ in colorectal carcinoma

Hongbo Zhang1, Jian Chen2, Jinchen Hu1, Xixun Wang1, Rongbao Jia1, Lixin Jiang1,()   

  1. 1. Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China
    2. Central Laboratory, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China
  • Received:2017-02-27 Published:2017-10-25
  • Corresponding author: Lixin Jiang
  • About author:
    Corresponding author:Jiang Lixin, Email:
引用本文:

张红波, 陈剑, 胡金晨, 王熙勋, 贾荣保, 姜立新. 结直肠癌组织中CyPJ的表达及其临床意义[J/OL]. 中华结直肠疾病电子杂志, 2017, 06(05): 387-391.

Hongbo Zhang, Jian Chen, Jinchen Hu, Xixun Wang, Rongbao Jia, Lixin Jiang. Expression and clinical significance of CyPJ in colorectal carcinoma[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(05): 387-391.

目的

观察亲环素J(CyPJ)在结直肠癌组织中的分布和表达,探讨其在结直肠癌发生发展中的临床意义。

方法

收集2015年5月至2016年4月期间青岛大学附属烟台毓璜顶医院胃肠外一科61例结直肠癌患者的手术切除的组织标本。采用免疫组织化学染色法检测CyPJ蛋白在61例结直肠癌组织及相应癌旁组织中的表达水平。应用SPSS 23.0统计学软件分析结直肠癌组织中CyPJ的蛋白表达率及其与结直肠癌分各种临床病理的关系。计数资料比较采用卡方检验或Fisher确切概率法,以P<0.05为差异具有统计学意义。

结果

CyPJ蛋白在结直肠癌组织表现为高表达,且癌旁组织也可见阳性表达,但颜色相对较浅。CyPJ蛋白的表达主要位于细胞质,少量表达于细胞核。结直肠癌组织及癌旁组织中CyPJ蛋白的阳性表达率分别为78.7%(48/61)、39.3%(24/61),两者比较,差异有统计学意义(χ2=19.520,P<0.001)。CyPJ蛋白在结直肠癌组织中的表达水平与TNM分期、肿瘤浸润深度及淋巴结转移比较,差异均有统计学意义(χ2=13.585,11.767,13.613,P均<0.001);而与其他临床病理因素如肿瘤大小、肿瘤部位、肿瘤分化程度性别、年龄、及有无远处转移比较,差异无统计学意义(χ2=1.010,0.061,0.119,1.130,2.646,0.416,P均>0.05)。

结论

CyPJ蛋白在结直肠癌组织中强表达,提示其可能与结直肠癌的发生、发展及转移密切相关。

Objective

To investigate the expression and distribution of CyPJ in the colorectal cancer (CRC) and to explore its clinical significance in the development and progression of CRC.

Methods

The clinical data and surgical specimens of 61 patients with colorectal carcinoma who were treated from May 2015 to April 2016 that were collected from the Department of Gastrointestinal Surgery, Qingdao University Affiliated Yantai Yuhuangding Hospital. Detected by immunohistochemical staining technique in 61 cases of CyPJ protein expression in colorectal carcinoma and paracarcinoma tissues levels. The relationship of the expression of CyPJ with the clinicopathological features of colorectal carcinoma was analyzed using SPSS 23. 0 statistical analysis software. The count data were evaluated using the chi-square test and Fisher exact probability. P<0. 05 was considered as significant difference.

Results

Expression of CyPJ protein in colorectal cancer tissues is overexpressed and the positive expression of paracarcinoma tissues is visible, but the color lighter. Expression of CyPJ protein is mainly located in the cytoplasm, with little distribution in the nucleus. The positive expression rates of CyPJ protein in the CRC and paracarcinoma tissues were 78. 7% (48/61)and 39. 3% (24/61), respectively, with a significant difference (χ2=19. 520, P<0. 05). The expression of CyPJ protein in patients with CRC was however associated with the TNM stage, depth of tumor invasion and lymph node metastasis (χ2=13. 585, 11. 767, 13. 613, P<0. 05), but No significant correlation was found between the expression of CyPJ and age, sex, tumor size, tumor site, tumor differentiation and distant metastasis (χ2=1. 010, 0. 061, 0. 119, 1. 130, 2. 646, 0. 416, P>0. 05).

Conclusion

CyPJ protein is overexpressed in colorectal carcinoma, suggests that it may be associated with the occurrence, development and metastasis of colorectal carcinoma.

表1 CyPJ蛋白在结直肠癌组织及癌旁正常组织中的表达差异(例)
图1 A图示CRC肿瘤组织中CyPJ蛋白阳性表达于胞浆,并且可见少量在细胞核周聚集,颜色加重;B图示癌旁组织中CyPJ蛋白阳性表达细胞浆和细胞核;C图示CRC肿瘤组织中CyPJ阴性表达;D图示癌旁组织中CyPJ阴性表达(SP×400)
表2 61例CRC患者临床病理因素与CYPJ蛋白阳性表达率的关系[例(%)]
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