运用孟德尔随机化分析的方法，探讨雄性激素水平与肛周脓肿发生的因果关联。

利用全基因关联研究的数据进行分析，暴露因素为男性和女性的总睾酮水平、可利用睾酮水平及性激素结合球蛋白水平，结局变量为肛周脓肿。采用逆方差加权法、MR-Egger法、加权中位数法进行孟德尔随机化分析，并进行异质性检验、敏感性分析、多效性分析。

男性可利用睾酮水平逆方差加权法，结果显示OR=1.0017，95%CI：1.0003~1.0030，异质性检验不存在异常变量，敏感性分析显示稳健，未发现水平多效性。

男性的生物可利用睾酮水平与肛周脓肿存在因果关系，男性及女性总睾酮水平、女性生物可利用睾酮水平、男性及女性的性激素结合球蛋白水平与肛周脓肿不存在因果关系。

To explore the causal association between levels of male hormones and the incidence of perianal abscesses, this study employs Mendelian randomization analysis.

Utilizing data from genome-wide association studies, the study considers total testosterone levels in males and females, bioavailable testosterone levels, and sex hormone-binding globulin levels as exposure variables, with the incidence of perianal abscesses serving as the outcome variable. Various Mendelian randomization methods, including inverse-variance weighted, MR-Egger, and weighted median techniques, were applied, complemented by heterogeneity tests, sensitivity analyses, and assessments for pleiotropy.

Employing inverse-variance weighted analysis, bioavailable testosterone levels in males revealed an odds ratio of 1.0017, 95% CI:1.0003~1.0030. Heterogeneity tests indicated no outlier variables, and sensitivity analyses confirmed the robustness of these findings, ruling out the presence of horizontal pleiotropy.

The study concludes that there is a causal relationship between bioavailable testosterone levels in males and the incidence of perianal abscesses; however, no such causality was observed for total testosterone levels in both genders, bioavailable testosterone levels in females, or sex hormone-binding globulin levels in either gender.