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中华结直肠疾病电子杂志

中华结直肠疾病电子杂志 ›› 2018, Vol. 07 ›› Issue (05) : 437 -441. doi: 10.3877/cma.j.issn.2095-3224.2018.05.006

所属专题： 文献；

论著

长非编码RNA H19在结直肠癌化疗敏感性中的作用研究
王秀梅1, 赵海平2,†(), 秦琼3, 刘永强4, 张兵2, 武永胜2   
  1. 1. 010020　呼和浩特，内蒙古自治区肿瘤医院&内蒙古医科大学附属人民医院肿瘤内科
    2. 010020　呼和浩特，内蒙古自治区肿瘤医院&内蒙古医科大学附属人民医院腹外科
    3. 017000　内蒙古鄂尔多斯达拉特旗人民医院肿瘤内科
    4. 010059　呼和浩特，内蒙古医科大学附属医院腹外科
  • 收稿日期:2017-09-17 出版日期:2018-10-25
  • 通信作者: 赵海平
  • 基金资助:
    内蒙古自治区肿瘤医院院内基金(No.2017066)

Role of long noncoding RNA H19 in chemosensitivity of colorectal cancer

Xiumei Wang1, Haiping Zhao2,†(), Qiong Qin3, Yongqiang Liu4, Bing Zhang2, Yongsheng Wu2   

  1. 1. Department of oncology, Inner Mongolia Cancer Hospital & Department of oncology, The Affiliated People′s Hospital of Inner Mongolia Medical University, Inner Mongolia 010020, China
    2. Department of General Surgery Inner Mongolia Cancer Hospital & Department of General Surgery, The Affiliated People′s Hospital of Inner Mongolia Medical University, Inner Mongolia 010020, China
    3. Department of Oncology, Inner Mongolia Ordos Dalad Banner People′s Hospital, Inner Mongolia 017000, China
    4. Department of General Surgery, The Affiliated People′s Hospital of Inner Mongolia Medical University, Inner Mongolia 010059, China
  • Received:2017-09-17 Published:2018-10-25
  • Corresponding author: Haiping Zhao
  • About author:
    Corresponding author: Zhao Haiping, Email:
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引用本文：

王秀梅, 赵海平, 秦琼, 刘永强, 张兵, 武永胜. 长非编码RNA H19在结直肠癌化疗敏感性中的作用研究[J/OL]. 中华结直肠疾病电子杂志, 2018, 07(05): 437-441.

Xiumei Wang, Haiping Zhao, Qiong Qin, Yongqiang Liu, Bing Zhang, Yongsheng Wu. Role of long noncoding RNA H19 in chemosensitivity of colorectal cancer[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2018, 07(05): 437-441.

目的

研究长非编码RNA H19在结直肠癌化疗药物敏感性中作用。

方法

分别构建lncRNA H19稳定过表达及表达沉默的结肠癌细胞株，分别加入5-FU培养，RT-qPCR检测H19表达，MTT法检测各组细胞增殖率，通过划痕实验检测各组迁移能力；Western blot检测各组细胞凋亡蛋白、耐药蛋白MDR1、MRP1和BCRP表达。

结果

与正常细胞比较，lncRNA H19在结直肠癌细胞中表达升高，lncRNA H19稳定过表达组细胞增殖率明显高于H19表达沉默组，lncRNA H19稳定过表达组细胞48 h迁移距离明显大于H19表达沉默组，lncRNA H19稳定过表达组Bax蛋白表达下调，BCL-2、MDR1、MRP1和BCRP表达上调，H19表达沉默组Bax蛋白表达上调，BCL-2、MDR1、MRP1和BCRP表达下调。

结论

lncRNA H19高表达的结直肠癌降低结直肠癌对化疗药物的敏感性，下调lncRNA H19表达可提高化疗药物的疗效。

关键词: 结直肠肿瘤, lncRNA H19, 化疗敏感性, 长链非编码RNA, 凋亡
Objective

To study the role of long non-coding RNA H19 in the chemosensitivity of colorectal cancer.

Methods

The colon cancer cell lines stably overexpressed and silenced with lncRNA H19 were constructed and cultured in 5-FU. The expression of H19 was detected by RT-qPCR. The cell proliferation rate was measured by MTT assay. The migration ability of each group was evaluated by scratch test. The protein expressions of MDR1, MRP1 and BCRP were detected by Western blot.

Results

Compared with normal cells, the expression of lncRNA H19 increased, and the proliferation rate of lncRNA H19 stable overexpression group was significantly higher than that of H19 silencing group. The migration distance in lncRNA H19 stable overexpression group was significantly longer than that in H19 silencing group and the overexpression of bcl-2 protein was down-regulated and bax、MDR1、MRP1 and BCRP was up-regulated in lncRNA H19 stable overexpression group. The expression of bcl-2 protein was up-regulated and the expression of bax、MDR1、MRP1 and BCRP was down-regulated in H19 silencing group.

Conclusion

Colorectal cancer with high expression of lncRNA H19 reduces the sensitivity of colorectal cancer to chemotherapeutic drugs, and down-regulation of lncRNA H19 expression can improve the curative effect of chemotherapy drugs.

Key words: Colorectal neoplasms, lncRNA H19, Chemosensitivity, Long Noncoding RNA, Apoptosis
图1 lncRNA H19在结直肠癌组织中高表达
图2 lncRNA H19转染
图3 结肠癌细胞株LoVo对5-FU的化疗敏感性
图4 各组细胞迁移水平
图5 蛋白表达印迹图
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