切换至 "中华医学电子期刊资源库"

中华结直肠疾病电子杂志 ›› 2017, Vol. 06 ›› Issue (06) : 488 -493. doi: 10.3877/cma.j.issn.2095-3224.2017.06.009

所属专题: 文献

论著

人催乳素受体蛋白在肛周脓肿患者中的表达及其与预后的关系
陶勇1, 杨关根1, 裘建明1, 徐侃1,(), 王东1, 汪鸿涛1   
  1. 1. 310009 杭州,浙江中医药大学附属杭州第三人民医院肛肠科
  • 收稿日期:2017-05-22 出版日期:2017-12-25
  • 通信作者: 徐侃
  • 基金资助:
    杭州市科技局重点专科专病项目(No.20160533B27;No.20150733Q28); 浙江省自然科学基金项目(No.LY15H030001;LY15H160001))

Association of prolactin receptor experssion in human perianal abscess with postoperative prognosis

Yong Tao1, Guangen Yang1, Jianming Qiu1, Kan Xu1,(), Dong Wang1, Hongtao Wang1   

  1. 1. Department of Colorectal Surgery, Zhejiang Chinese Medicine University Affiliated No.3 Hangzhou Hospital, Hangzhou 310009, China
  • Received:2017-05-22 Published:2017-12-25
  • Corresponding author: Kan Xu
  • About author:
    Corresponding author: Xu Kan, Email:
引用本文:

陶勇, 杨关根, 裘建明, 徐侃, 王东, 汪鸿涛. 人催乳素受体蛋白在肛周脓肿患者中的表达及其与预后的关系[J/OL]. 中华结直肠疾病电子杂志, 2017, 06(06): 488-493.

Yong Tao, Guangen Yang, Jianming Qiu, Kan Xu, Dong Wang, Hongtao Wang. Association of prolactin receptor experssion in human perianal abscess with postoperative prognosis[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(06): 488-493.

目的

探讨人催乳素受体蛋白在肛周脓肿组织中的表达及其与临床病理特征的关系,预测可能作为肛周脓肿预后的一个生物标志物。

方法

收集2015年08月至2016年08月杭州市第三人民医院肛肠外科收治的肛周脓肿行手术切排并经病理证实的60例患者的肛管黏膜组织标本(肛周脓肿组)及临床资料。另取年龄、性别、体重相匹配的8例单纯痔病患者的肛管黏膜组织标本作为对照(对照组)。运用免疫组织化学(免疫组化)和Western blot方法检测两组标本中催乳素受体蛋白表达水平,并结合临床病理资料进行统计分析。同时采用单因素及多因素Logistic回归分析探讨组织中PRLR表达水平与肛周脓肿预后的相关性。

结果

免疫组化结果显示,PRLR蛋白阳性的棕色颗粒主要弥漫性分布在细胞膜中,部分可见于细胞质。其中在脓肿组肛管黏膜中低表达率为68.33%(41/60)及高表达率31.67%(19/60),与对照痔病组PRLR蛋白高表达相比,差异具有统计学意义(χ2=5.701,P<0.05);同时Western Blot结果显示PRLR蛋白在脓肿组中的表达灰度比值(0.515±0.067)明显低于对照组灰度比值(0.836±0.035)(t=3.14,P<0.01);全组患者未愈为34例,占56.67%。其中PRLR低表达与高表达,分别为79.41%(27/41)和20.59%(7/19)。单因素及多因素分析显示,脓肿性质(OR=7.911,95% CI:1.115~20.161,P=0.039)、脓肿切口(OR=3.246,95%CI:0.774~5.742,P=0.042)及PRLR低表达量(OR=9.099,95%CI:1.727~14.426,P=0.016)是肛周脓肿患者预后不良的独立危险因素(均P<0.05)。

结论

PRLR蛋白在肛周脓肿患者肛管黏膜组织中表达显著降低,同时低表达的PRLR对判断手术后肛周脓肿患者预后具有较好的参考价值。

Objective

To investigate the association of prolactin receptor (PRLR) expression in human perianal abscess with its prognosis,which may be a new prognosis biomarker of perianal abscess-related.

Methods

Clinical data and resection samples of 60 patients with perianal abscess identified by pathology undergoing resection in our department from August 2015 to August 2016 were collected as well as 8 patients receiving only hemorroidectomy (control group), matching with perianal abscess group in age, gender and body weight. The PRLR expression in perianal abscess tissues and matched nonabscess tissues collected was detected by immunohistochemical staining and Western blotting. The associations of PRLR level with overall prognosis,clinicopathological features were analyzed, univariate and multivariate logistic regression analysis.

Results

Immunohistochemical staining showed that the positive brown granules were mainly distributed on the cell membrane,and also in cytoplasm partially. And the low expression of PRLR protein in perianal abscess group was 81.67% (41/60) and high espression wsa 31.67% (19/60), which was significantly higher than that of control group (P<0.05). The relative gray value of Western blotting in perianal abscess tissues were 0.515±0.067, which were significantly lower than those of matched nonabscess tissues (0.836±0.035 and P<0.05). Patients with low expression of PRLR protein had poor prognosis than those with low expression (68.85% vs. 36.84%, P< 0.05). Univariate and Multivariate logistic regression analysis showed that the complexity of abscess (OR=7.911, 95%CI: 1.115~20.161, P=0.039), the number of surgical incision (OR=3.246, 95%CI: 0.774~5.742, P=0.042) and the expression of PRLR protein (OR=9.099, 95%CI: 1.727~14.426, P=0.016) were independent risk factors for poor prognosis of patients with perianal abscess (all P<0.05).

Conclusions

PRLP expression in perianal abscess patient was low, which may be a valuable in predicting prognosis.

图1 免疫组化检测PRLR结果×100(箭头指示):1A肛周脓肿患者肛管黏膜标本高表达;1B肛周脓肿患者肛管黏膜标本低表达;1C痔疮患者肛管黏膜标本高表达;1D痔疮患者肛管黏膜标本低表达
图2B 两组患者Western blot检测PRLR蛋白电泳结果
表1 PRLR表达与肛周脓肿患者临床病理特征的关系
图3 PRLR高表达与低表达总体治愈率的比较
表2 PRLR表达与肛周脓肿预后的单因素关系
表3 PRLR表达与肛周脓肿预后的多因素关系
1
Ommer A,Herold A,Berg E, et al.German S3 guidelines: anal abscess and fistula (second revised version) [J]. Langenbecks Arch Surg, 2017, 402(2): 191-201.
2
Sahnan K,Adegbola SO,Tozer PJ, et al.Perianal abscess [J]. BMJ, 2017, 2(21): 365- 475.
3
Soto L,Lagos AF,Isla A, et al.Immunostimulatory effects of prolactin on TLR1 and TLR5M in SHK-1 cells infected with Piscirickettsia salmonis [J]. Dis Aquat Organ, 2016, 118(3): 237-245.
4
Marinelli C,Bertalot T,Zusso M, et al.Systematic Review of Pharmacological Properties of the Oligodendrocyte Lineage [J]. Front Cell Neurosci, 2016, 12(2): 10-27.
5
Ueda EK,Huang K,Nguyen V, et al.Distribution of prolactin receptors suggests an intraductal role for prolactin in mouse and human mammary gland, a finding supported by analysis of signaling in polarized monolayer cultures [J]. Cell Tissue Res, 2011, 346(2): 175-189.
6
Shelly S,Boaz M,Orbach H.Prolactin and autoimmunity [J]. Autoimmun Rev, 2012 , 11(6-7): A465-470.
7
Liu Y,Zhang Y,Jiang J, et al.GHR/PRLR heteromultimer is composed of GHR homodimers and PRLR homodimers [J]. Mol Endocrinol, 2016, 30(5): 504-514.
8
Morammazi S,Masoudi AA,Vaze Torshizi R, et al.Changes in the expression of the prolactin receptor(PRLR) gene in different physiological stages in the mammary gland of the Iranian Adani Goat [J]. Reprod Domest Anim, 2016, 51(4): 585-590.
9
Tang MW,Reedquist KA,Garcia S, et al.The prolactin receptor is expressed in rheumatoid arthritis and psoriatic arthritis synovial tissue and contributes to macrophage activation [J]. Rheumatology (Oxford), 2016, 55(12): 2248-2259.
10
López-Rincón G,Mancilla R,Pereira-Suárez AL, et al.Expression of autocrine prolactin and the short isoform of prolactin receptor are associated with inflammatory response and apoptosis in monocytes stimulated with mycobacterium bovis proteins [J]. Exp Mol Pathol, 2015, 98(3): 517-526.
11
Pereira Suarez AL, et al.Prolactin in inflammatory response [J]. Adv Exp Med Biol, 2015, 10(7): 243-264.
12
Savino W.Prolactin: An immunodulator in health and disease [J]. Front Horm Res, 2017, 48(2): 69-75.
13
López-Rincón G,Pereira-Suárez AL,Del Toro-Arreola S, et al.Lipopolysaccharide induces the expression of an autocrine prolactin loop enhancing inflammatory response in monocytes [J]. J Inflamm (Lond), 2013, 10(1): 24.
14
Yang L,Zhang Y.Tumor-associated macrophages: from basic research to clinical application [J]. J Hematol Oncol, 2017, 10(1): 58.
15
Feuerstein R,Seidl M,Prinz M,Henneke P.MyD88 in macrophages is critical for abscess resolution in staphylococcal skin infection [J]. J Immunol, 2015, 194(6): 2735-2745.
16
Soto L,Lago AF,IsIa A,Haussmann D, et al.Immunostimulatory effects of prolactin on TLR1 and TLR5M in SHK-1 cells infected with Piscirickettsia salmonis [J]. Dis Aquat Organ, 2016, 118(3): 237-245.
17
Goffin V,Touraine P.The prolactin receptor as a therapeutic target in human diseases: browsing new potential indications [J]. Expert Opin Ther Targets, 2015, 19(9): 1229-1244.
18
Benedetto N,Auriault C.Prolactin-cytokine network in the defence against acanth amoeba castellanii in murine microglia [J]. Eur Cytokine Netw, 2002, 13(4): 447-455.
[1] 张晓宇, 殷雨来, 张银旭. 阿帕替尼联合新辅助化疗对三阴性乳腺癌的疗效及预后分析[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 346-352.
[2] 许杰, 李亚俊, 韩军伟. 两种入路下腹腔镜根治性全胃切除术治疗超重胃癌的效果比较[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 19-22.
[3] 高杰红, 黎平平, 齐婧, 代引海. ETFA和CD34在乳腺癌中的表达及与临床病理参数和预后的关系研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 64-67.
[4] 李代勤, 刘佩杰. 动态增强磁共振评估中晚期低位直肠癌同步放化疗后疗效及预后的价值[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 100-103.
[5] 屈翔宇, 张懿刚, 李浩令, 邱天, 谈燚. USP24及其共表达肿瘤代谢基因在肝细胞癌中的诊断和预后预测作用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 659-662.
[6] 顾雯, 凌守鑫, 唐海利, 甘雪梅. 两种不同手术入路在甲状腺乳头状癌患者开放性根治性术中的应用比较[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 687-690.
[7] 付成旺, 杨大刚, 王榕, 李福堂. 营养与炎症指标在可切除胰腺癌中的研究进展[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 704-708.
[8] 梁孟杰, 朱欢欢, 王行舟, 江航, 艾世超, 孙锋, 宋鹏, 王萌, 刘颂, 夏雪峰, 杜峻峰, 傅双, 陆晓峰, 沈晓菲, 管文贤. 联合免疫治疗的胃癌转化治疗患者预后及术后并发症分析[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 619-623.
[9] 关小玲, 周文营, 陈洪平. PTAAR在乙肝相关慢加急性肝衰竭患者短期预后中的预测价值[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 841-845.
[10] 张润锦, 阳盼, 林燕斯, 刘尊龙, 刘建平, 金小岩. EB病毒相关胆管癌伴多发转移一例及国内文献复习[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 865-869.
[11] 陈晓鹏, 王佳妮, 练庆海, 杨九妹. 肝细胞癌VOPP1表达及其与预后的关系[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 876-882.
[12] 中国抗癌协会, 中国抗癌协会大肠癌专业委员会. 中国恶性肿瘤整合诊治指南-肛管癌(2024 版)[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 441-449.
[13] 韩加刚, 王振军. 梗阻性左半结肠癌的治疗策略[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 450-458.
[14] 王景明, 王磊, 许小多, 邢文强, 张兆岩, 黄伟敏. 腰椎椎旁肌的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(09): 846-852.
[15] 郭曌蓉, 王歆光, 刘毅强, 何英剑, 王立泽, 杨飏, 汪星, 曹威, 谷重山, 范铁, 李金锋, 范照青. 不同亚型乳腺叶状肿瘤的临床病理特征及预后危险因素分析[J/OL]. 中华临床医师杂志(电子版), 2024, 18(06): 524-532.
阅读次数
全文


摘要