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中华结直肠疾病电子杂志

中华结直肠疾病电子杂志 ›› 2017, Vol. 06 ›› Issue (02) : 121 -126. doi: 10.3877/cma.j.issn.2095-3224.2017.02.008

所属专题： 文献；

论著

慢性功能性便秘患者的肠道菌群分析
黄林生1, 高仁元2, 严雪冰2, 祝琦2, 潘成2, 尹明明2, 李豪2, 孔程2, 张鹏2, 沈通一2, 秦环龙1,†()   
  1. 1. 200072　上海，安徽医科大学上海十院临床学院；200072　上海，同济大学附属第十人民医院普外科；200072　上海，同济大学医学院肠道疾病研究所
    2. 200072　上海，同济大学附属第十人民医院普外科；200072　上海，同济大学医学院肠道疾病研究所
  • 收稿日期:2016-12-19 出版日期:2017-04-25
  • 通信作者: 秦环龙
  • 基金资助:
    国家自然科学基金项目(No.81230057；No.81472262；No.81302066); 上海新兴前沿技术联合攻关项目(No.SHDC12012106)

Structure analysis of the gut microbiota in chronic functional constipation patients

Linsheng Huang1, Renyuan Gao2, Xuebing Yan2, Qi Zhu2, Cheng Pan2, Mingming Yin2, Hao Li2, Cheng Kong2, Peng Zhang2, Tongyi Shen2, Huanlong Qin1,†()   

  1. 1. Shanghai Tenth People′s Hospital Clinical College of Anhui Medical University; Department of General Surgery, Shanghai Tenth People′s Hospital, Tongji University School of Medicine; Research Institute for Intestinal Diseases of Tongji University School of Medicine, Shanghai 200072, China
    2. Department of General Surgery, Shanghai Tenth People′s Hospital, Tongji University School of Medicine; Research Institute for Intestinal Diseases of Tongji University School of Medicine, Shanghai 200072, China
  • Received:2016-12-19 Published:2017-04-25
  • Corresponding author: Huanlong Qin
  • About author:
    Correspondence author: Qin Huanlong, Email:
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引用本文：

黄林生, 高仁元, 严雪冰, 祝琦, 潘成, 尹明明, 李豪, 孔程, 张鹏, 沈通一, 秦环龙. 慢性功能性便秘患者的肠道菌群分析[J]. 中华结直肠疾病电子杂志, 2017, 06(02): 121-126.

Linsheng Huang, Renyuan Gao, Xuebing Yan, Qi Zhu, Cheng Pan, Mingming Yin, Hao Li, Cheng Kong, Peng Zhang, Tongyi Shen, Huanlong Qin. Structure analysis of the gut microbiota in chronic functional constipation patients[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(02): 121-126.

目的

探讨慢性功能性便秘患者与健康对照组之间肠道菌群的差异。

方法

选取便秘组和健康对照组共计20人，留取新鲜粪便样本，冰块运送至实验室并存放于-80度冰箱。采用16S rRNA测序技术检测V4区鉴定菌群。

结果

发现两组人群的菌群丰富度差异有统计学意义（P<0.05），菌群多样性差异无统计学意义。便秘患者与健康对照组之间的肠道细菌组成在门和属水平差异均存在显著统计学意义（P<0.05）。在门水平，便秘患者肠道中放线菌门（Actinobacteria）丰度显著增加（Z=-3.10，P<0.05），而变形菌门（Proteobacteria）丰度显著降低（Z=-2.42，P<0.05），在属水平发现20个菌属差异存在统计学意义（P<0.05）。其中，Coprobacillus（Z=-2.10，P<0.05）、Hungatella（Z=-2.31，P<0.05）、Holdemanella（Z=-2.32，P<0.05）、Anaerostipes（Z=-3.78，P<0.01）、Bacteroidales_S24-7_group_norank（Z=-2.15，P<0.05）、Bacteroides（Z=-3.78，P<0.01）、Blautia（Z=-3.78，P<0.05）、Collinsella（Z=-3.02，P<0.05）、Dorea（Z=-2.95，P<0.05）、［Eubacterium］_ruminantium_group（Z=-2.70，P<0.05）、Erysipelotrichaceae_UCG-003（Z=-3.10，P<0.05）、Ruminococcaceae_uncultured（Z=-2.50，P<0.05）、Parabacteroides（Z=-2.87，P<0.05）菌属在便秘患者的肠道中丰度明显增多，而Megasphaera（Z=-2.31，P<0.05）、Paraprevotella（Z=-2.94，P<0.05）、Prevotella_2（Z=-3.10，P<0.05）、Prevotella_9（Z=-3.68，P<0.01）、Enterococcus（Z=-2.27，P<0.05）、Catenibacterium（Z=-2.48，P<0.05）、Clostridium_sensu_stricto_1（Z=-2.50，P<0.05）菌属在健康对照组的肠道中丰度明显增多。

结论

慢性功能性便秘患者的肠道菌群与健康人群的菌群存在较大差异，通过改变肠道菌群可能成为治疗慢性功能性便秘的新策略。

关键词: 便秘, 肠道菌群失调, 菌群丰度, 16S rRNA
Objective

To investigate the differences of gut microbiota between patients with chronic functional constipation (CFC) and health controls (HC).

Methods

16S rRNA highthroughout pyrosequencing on V4 region was employed to examine the difference of gut microbiota profiles between 10 CFC patients and 10 HCs. The obtained data were analyzed using bioinformatics.

Results

The richness analysis suggested that HCs have richer gut microbiota than CFC patients (all P<0.05), while no significant difference was observed in the diversity analysis.There was a significant structure difference of gut microbiota between CFC patients and HCs both at the phylum and genus levels (all P<0.05). At the phylum level, Actinobacteria in CFC patients is richer than that in HCs, but Proteobacteria is opposite (all P<0.05). At the genus level, 20 gut microbiota were found to have a changed relative abundance between CFC patients and HCs, in which Coprobacillus, Hungatella, Holdemanella, Anaerostipes, Bacteroidales_S24-7_group_norank, Bacteroides, Blautia, [Eubacterium]_ruminantium_group, Collinsella, Dorea, Erysipelotrichaceae_UCG-003, Parabacteroides and Ruminococcaceae_uncultured were increased in CFC patients while Megasphaera, Paraprevotella, Prevotella_2, Prevotella_9, Enterococcus, Catenibacterium and Clostridium_sensu_stricto_1 were increased in HCs.

Conclusion

There was a significant structure difference of gut microbiota between CFC patients and HCs. Therefore, targeting the gut microbiota dysbiosis may be a promising therapeutic strategy for CFC patients.

Key words: Constipation, Intestinal flora dysregulation, Bacteria abundance, 16S rRNA, richness
表1 人群的基本信息（±s）
组别 例数 年龄（岁） 身高（cm） 体重（Kg） BMI指数（Kg/m2） 每周排便次数（次） 便秘时间（月）
便秘组 10 71.00±4.89 168.70±2.31 63.20±3.24 22.13±0.82 1.80±0.21 105.6 0±35.95
健康组 10 71.40±0.67 166.40±2.06 68.30±2.42 24.64±0.65 6.70±0.15 0.00
Z值 ? t=0.08 t=0.74 t=1.26 t=2.40 t=5.60 t=2.90
P值 ? P=0.93 P=0.45 P=0.22 P=0.03 P<0.01 P=0.01
表2 CFC和HC在属水平显著差异菌群的相对丰度（%）
组别 Parabacteroides Prevotella_9 Bacteroides
便秘组 7.92±2.53 1.10±0.77 42.75±3.76
健康组 1.19±0.47 42.21±9.04 9.27±3.20
Z值 -2.87 -3.68 -3.78
P值 0.0029 <0.01 <0.01
比值（Log2） 0.57 5.25 0.36
图1 图示便秘组和健康对照组之间丰度（Ace和Chao指数）和多样性（Shannon和Simpson指数）差异*P<0.05
表3 CFC和HC在门水平菌群的相对丰度比较（%）
组别 Tenericutes Proteobacteria Firmicutes Bacteroidetes Actinobacteria Fusobacteria
便秘组 0.18±0.12 2.52±0.94 38.81±3.37 57.58±3.28 0.71±0.16 0.03±0.02
健康组 0.23±0.10 9.80±3.91 28.98±5.76 59.89±5.37 0.11±0.03 0. 99±0.79
Z值 -1.27 -2.42 -1.81 -0.76 -3.10 -1.56
P值 >0.05 0.015 >0.05 >0.05 0.0288 >0.05
图2 便秘组和健康对照组之间放线菌门（Actinobacteria）和变形菌门（Proteobacteria）的丰度差异*P<0.05
图3 便秘组和健康组菌群在属水平的Heatmap图
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