Expression of Stathmin and Cathepsin B in colorectal cancer

doi:10.3877/cma.j.issn.2095-3224.2014.03.05

Abstract

Abstract:

Objective

To investigate the differential expression of Stathmin and Cathepsin B in human colorectal cancer tissues and related metastatic foci.And to discuss the clinicopathological significance of these two markers.

Methods

Immunohistochemical staining for Stathmin and Cathepsin B was performed in the colorectal cancer tissues of 158 cases and 64 related metastatic foci.

Results

Both Stathmin and Cathepsin B are upregulated in colorectal cancer and related metastatic samples.The location of Stathmin and Cathepsin B transferred from cytoplasm to nuclear in low differentiation colorectal cancer samples compared with high and mediate differentiation samples.

Conclusion

Stathmin and Cathepsin B might be valuable biomarkers for clinical differentiation of colorectal cancer.

Key words: Colorectal neoplasms, Egg white, Immunohistochemistry

Zheng-duo YANG, Dan ZHANG, Hong-li LU, Li ZHANG, Hui MA, Gang WANG, Shi-wu ZHANG. Expression of Stathmin and Cathepsin B in colorectal cancer[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2014, 03(03): 180-184.

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Figures/Tables 3

References 22

[1]	Jemal A,Bray F,Center MM, et al.Global cancer statistics.CA, 2011, 61(2): 69-90.
[2]	Mistry SJ,Atweh GF.Role of stathmin in the regulation of the mitotic spindle：potential applications in cancer therapy.The Mount Sinai journal of medicine, 2002, 69(5): 299-304.
[3]	Alli E,Yang JM,Hait WN.Silencing of stathmin induces tumor－suppressor function in breast cancer cell lines harboring mutant p53.Oncogene, 2007, 26(7): 1003-1012.
[4]	Zheng P,Liu YX,Chen L, et al.Stathmin, a new target of PRL－3 identified by proteomic methods, plays a key role in progression and metastasis of colorectal cancer.Journal of proteome research, 2010, 9(10): 4897-4905.
[5]	Byrne FL,Yang L,Phillips PA, et al.RNAi－mediated stathmin suppression reduces lung metastasis in an orthotopic neuroblastoma mouse model.Oncogene, 2014, 33(7): 882-890.
[6]	Zhang CY,Xiao ZA,Zeng YC, et al.Expression of stathmin mRNA and protein in laryngeal squamous cell carcinoma and its clinical implication.Zhonghua er bi yan hou tou jing wai ke za zhi, 2008, 43(4): 291-295.
[7]	Sadow PM,Rumilla KM,Erickson LA, et al.Stathmin expression in pheochromocytomas, paragangliomas, and in other endocrine tumors.Endocrine pathology, 2008, 19(2): 97-103.
[8]	Wei SH,Lin F,Wang X, et al.Prognostic significance of stathmin expression in correlation with metastasis and clinicopathological characteristics in human ovarian carcinoma.Acta histochemica, 2008, 110(1): 59-65.
[9]	Wang C,Sun B,Zhou Y, et al.Cathepsin B degrades amyloid－beta in mice expressing wild－type human amyloid precursor protein.The Journal of biological chemistry, 2012, 287(47): 39834-39841.
[10]	Kindy MS,Yu J,Zhu H, et al.Deletion of the cathepsin B gene improves memory deficits in a transgenic ALZHeimer's disease mouse model expressing Abetapp containing the wild－type beta－secretase site sequence.JAD, 2012, 29(4): 827-840.
[11]	Rafn B,Nielsen CF,Andersen SH, et al.ErbB2－driven breast cancer cell invasion depends on a complex signaling network activating myeloid zinc finger－1－dependent cathepsin B expression.Molecular cell, 2012, 45(6): 764-776.
[12]	Andl CD,McCowan KM,Allison GL, et al.Cathepsin B is the driving force of esophageal cell invasion in a fibroblast－dependent manner.Neoplasia, 2010, 12(6): 485-498.
[13]	Bao W,Fan Q,Luo X, et al.Silencing of Cathepsin B suppresses the proliferation and invasion of endometrial cancer.Oncology reports, 2013, 30(2): 723-730.
[14]	Chan AT,Baba Y,Shima K, et al.Cathepsin B expression and survival in colon cancer：implications for molecular detection of neoplasia.Cancer epidemiology, biomarkers & prevention：a publication of the american association for cancer research, cosponsored.The american society of preventive oncology, 2010, 19(11): 2777-2785.
[15]	Sun B,Zhang S,Zhang D, et al.Identification of metastasis－related proteins and their clinical relevance to triple－negative human breast cancer.Clinical cancer research, 2008, 14(21): 7050-7059.
[16]	Sellin ME,Holmfeldt P,Stenmark S, et al.Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin.Molecular biology of the cell, 2008, 19(7): 2897-2906.
[17]	Yuan RH,Jeng YM,Chen HL, et al.Stathmin overexpression cooperates with p53 mutation and osteopontin overexpression, and is associated with tumour progression, early recurrence, and poor prognosis in hepatocellular carcinoma.The Journal of pathology, 2006, 209(4): 549-558.
[18]	Belletti B,Nicoloso MS,Schiappacassi M, et al.Stathmin activity influences sarcoma cell shape, motility, and metastatic potential.Molecular biology of the cell, 2008, 19(5): 2003-2013.
[19]	Singer S,Malz M,Herpel E, et al.Coordinated expression of stathmin family members by far upstream sequence element－binding protein－1 increases motility in non–small cell lung cancer.Cancer research, 2009, 69(6): 2234-2243.
[20]	Carney BK,Cassimeris L. Stathmin/oncoprotein 18, a microtubule regulatory protein, is required for survival of both normal and cancer cell lines lacking the tumor suppressor, p53.Cancer biology & therapy, 2010, 9(9): 699-709.
[21]	Alapati K,Kesanakurti D,Rao JS, et al.UPAR and cathepsin B－mediated compartmentalization of JNK regulates the migration of glioma－initiating cells.Stem cell research, 2014, 12(3): 716-729.
[22]	Ma W,Ma L,Zhe H, et al.Detection of esophageal squamous cell carcinoma by cathepsin B activity in nude mice.PloS one, 2014, 9(3): 92351.

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