挖掘结肠癌肝转移过程中的核心基因模块和分子靶点，并验证其对临床预后及结肠癌转移能力的影响。

基于GEO数据库结肠癌肝转移测序样本，利用权重基因共表达网络分析（WGCNA）技术筛选转移相关基因模块。利用MCODE软件进一步挖掘结肠癌肝转移相关核心子模块并分析其功能。基于TCGA数据库，进行子模块基因对结肠癌预后影响的大临床样本验证。分子生物学方法验证子模块基因对结肠癌细胞系HCT116迁移和侵袭能力的影响。

WGCNA分析筛选出5个基因模块，其中模块1与结肠癌肝转移关系密切。模块1共包含4个核心子模块，主要参与G蛋白偶联受体信号调节、表观遗传学调控、mRNA的剪接调节等功能。大临床样本验证发现子模块4中的FOXC1基因与结肠癌患者生存率密切相关。敲减FOXC1在HCT116细胞中的表达后，HCT116的迁移能力（t=3.123，P=0.035）和侵袭能力（t=2.936，P=0.043）受到显著抑制。

本研究筛选出的结肠癌肝转移相关子模块可能具有重要的促转移作用，子模块基因FOXC1与结肠癌患者较差的生存率相关并具有促结肠癌转移能力。

To explore the core gene modules and molecular target in the process of colorectal cancer liver metastasis and to verify its impact on clinical prognosis and colorectal cancer metastasis ability.

Based on the sequencing samples of colorectal cancer liver metastasis in GEO database, WGCNA was used to screen metastasis-related gene modules. Using MCODE software to further explore the core sub-modules and analyze their function. TCGA database was used to analysis the effect of the target gene on the survival rate of colorectal cancer patients. Using molecular biology methods to verify the effect of sub-module genes on the migration and invasion of HCT116.

A total of 5 modules were identified, and module 1 was closely related to colorectal cancer liver metastasis. Module 1 included 4 core sub-modules. The function of the sub-modules included adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway, epigenetic regulation, mRNA splicing and so on. Large clinical sample verification found that the FOXC1 gene in sub-module 4 was closely related to the survival rate of colon cancer patients. After knocking down the expression of FOXC1 in HCT116 cells, the migration (t=3.123, P=0.035) and invasion (t=2.936, P=0.043) of HCT116 was significantly inhibited.

The sub-modules related to liver metastasis of colon cancer screened in this study may have an important role in promoting metastasis. The sub-module gene FOXC1 is associated with the poor prognosis of colon cancer patients and promotes colon cancer metastasis.