Effect of Changyian on the expression of nuclear factor kappa B and interleukin-10 in ulcerative colitis rats

doi:10.3877/cma.j.issn.2095-3224.2017.02.007

Abstract

Abstract:

Objective

To observe the effect of Changyian on the expression of NF-κB p65 and interleukin-10 in ulcerative colitis rats.

Methods

Ulcerative colitis rat model was copied by 2, 4, 6-TNBS.Thirty-nine rats were randomly divided into 3 groups: model group, Changyian group and salazosulfapyridine (SASP) group. After 21 days′ treatment, the changes of colonic mucosal damage index (CMDI) , levels of NF-κB p65 and IL-10 were observed.

Results

After 21 days′ treatment, the CMDI and the expression of NF-κB p65 of Changyian group was significantly lower than the model group (t=58.28, P=0.003; t=6.01, P=0.016) and SASP group (t=-22.10, P=0.026; t=-2.80, P=0.038) . The expression of interleukin-10 was obviously higher than that of model group and SASP group (t=-403.56, P=0.022; t=35.76, P=0.036) .

Conclusion

Changyian has good repair effect on ulcerative colitis rats colon mucosa. Its mechanism may be related to reduce the expression of NF-κB p65and improve the level of IL-I0.

Key words: Colitis, ulcerative, IL-I0, Nuclear factor kappa B p65, Changyian

Chunhua Ma, Jingyi Luo, Donghua Zhu, Shujuan Jing, Yi Shang. Effect of Changyian on the expression of nuclear factor kappa B and interleukin-10 in ulcerative colitis rats[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(02): 117-120.

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URL: https://zhjzcjbdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-3224.2017.02.007

https://zhjzcjbdzzz.cma-cmc.com.cn/EN/Y2017/V06/I02/117

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References 18

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分数	损伤程度
0	无损伤
1	轻度充血、水肿，表面光滑，无糜烂或溃疡
2	充血水肿，粘膜粗糙呈颗粒状，有糜烂或肠粘连
3	高度充血水肿，粘膜表面有坏死及溃疡形成，溃疡最大纵径<10 mm，肠壁增厚或表面有坏死及炎症
4	在3分基础上溃疡最大纵径>10 mm，或全肠壁坏死

分数	损伤程度
0	无损伤
1	轻度充血、水肿，表面光滑，无糜烂或溃疡
2	充血水肿，粘膜粗糙呈颗粒状，有糜烂或肠粘连
3	高度充血水肿，粘膜表面有坏死及溃疡形成，溃疡最大纵径<10 mm，肠壁增厚或表面有坏死及炎症
4	在3分基础上溃疡最大纵径>10 mm，或全肠壁坏死

组别	例数	CMDI评分	NF-κB p65表达	IL-10表达
肠易安汤组	13	0.88±0.12	0.17±0.38*	73744.49±348.88*
模型组	13	3.60±0.12	0.40±0.13	34673.11±11.65
SASP组	13	1.86±0.11	0.32±0.19*	55529.49±1803.25*
整体比较：F/P	?	7.63，<0.001	5.28，<0.001	34.28，<0.001
多重比较：LSD—t/P	肠易安汤组vs模型组	58.28，0.003	6.01，0.016	-403.56，0.022
?	肠易安汤组vs SASP组	-22.10，0.026	-2.80，0.038	35.76，0.036
?	模型组vs SASP组	-38.11，0.002	-1.27，0.008	41.70，0.018

组别	例数	CMDI评分	NF-κB p65表达	IL-10表达
肠易安汤组	13	0.88±0.12	0.17±0.38*	73744.49±348.88*
模型组	13	3.60±0.12	0.40±0.13	34673.11±11.65
SASP组	13	1.86±0.11	0.32±0.19*	55529.49±1803.25*
整体比较：F/P	?	7.63，<0.001	5.28，<0.001	34.28，<0.001
多重比较：LSD—t/P	肠易安汤组vs模型组	58.28，0.003	6.01，0.016	-403.56，0.022
?	肠易安汤组vs SASP组	-22.10，0.026	-2.80，0.038	35.76，0.036
?	模型组vs SASP组	-38.11，0.002	-1.27，0.008	41.70，0.018

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