To investigate the effect of potato extract with radiotherapy on the proliferation of colorectal cancer cells and its mechanism.

Human colorectal cancer cell line LS-174T was used to give different doses of X-ray irradiation and different concentrations of potato extracts. MTS method was used to detect cell proliferation and tumor inhibition rate in each group, Western blot method to detect Bcl-2, Caspase-3 The expression of protein in each group of cells, Annexin V-FITC/PI double staining flow cytometry was used to detect cell apoptosis in each group.

When LS-174T cells were treated with potato extract alone, which the concentration reached 25 μL/mL, the inhibitory effect on cell proliferation gradually increased. Especially, the inhibition of cell proliferation is most obvious with potato extract 50 μL/mL incubated for 72 hours (t=28.6, P=0.001). When the concentration of potato extract reaches 25 μL/mL combined with radiotherapy, the cell proliferation ability is obviously inhibited. After radiotherapy 8 Gy combined with 50 μL/mL of potato extract was treated on LS-174T cells for 72 h, the cell proliferation inhibited (t=12.1, P=0.007). Potato extract combined with radiotherapy reduced the expression of Bcl-2 protein in LS-174T cells, and the expression of Caspase-3 protein was high. Flow cytometry test showed that 50 μL/mL of potato extract combined with radiotherapy 8 Gy can promote cell apoptosis (t=18.3, P=0.003).

Potato extract combined with radiotherapy can significantly inhibit the proliferation of colorectal cancer cells, and its mechanism of action may be through mediating cell apoptosis, providing a new idea for the study of colorectal cancer radiosensitizers.