Functions and regulation of miR-10b in Uyger colorectal cancer metastasis

doi:10.3877/cma.j.issn.2095-3224.2017.05.006

Abstract

Abstract:

Objective

To investigate the role of miR-10b in metastasis of colon cancer of Uyger patients.

Methods

miR-10b expression was tested on CRC samples which were divided into metastatic groups and non-metastic groups of Uyger petients.The miR-10b-overexpressed or down-regulated SW620 cells were used to test the effects of miR-10b on invasion and migration of SW620 cells and to observe how miR-10b regulating KLF4. The miR-10b/klf4-overexpressed or down-regulated SW620 cells were used to test E-cadherin, cyclins D1 and p53 expression to test the involved mechanism in the regulatory function of miR-10b on metastasis and proliferation in CRC cells.

Results

miR-10b was over-expressed in metastatic CRC samples, compared with non-metastic ones of Uyger patients (t=-5.372, P<0.005). Inhibition of miR-10b in SW620 cells led to a notable reduction in invasion and migration assay compared with control cells (t=26.56, 10.40, P<0.05). We transfected miR-10b inhibitor into SW620 cells, an in crease of KLF4 mRNA level and protein level in SW620 cells was observed (t=3.78, P<0.05). miR-10b knockdown and KLF4 overexpression upregulated E-cadherin expression. Correlated with the modulated expression of miR-10b: the inhibition of miR-10b caused downegulation of cyclins D1 and p53, which were partly abrogated after co-transfecting with miR-10b and siRNA KLF4.

Conclusions

Expression of miR-10b is up-regulated in the metastasis CRC tissues and cells. miR-10b controls the metastasis and proliferation of CRC cells. miR-10b may exert its effect on metastasis and prolieration of CRC cells by regulating the expression of KLF4.miR-10b appears to modulate several independent signaling pathways that involved EMT, cell cycle and apoptosis.

Key words: Colorectal neoplasms, Neoplasm metastasis, miR-10b, Uyger

Shasha Su, Xiaolei He, Feng Gao. Functions and regulation of miR-10b in Uyger colorectal cancer metastasis[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(05): 381-386.

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Figures/Tables 6

References 27

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分组	例数	表达均值	标准差
非转移组	16	0.9944	0.69116
转移组	14	2.8236	1.14616
t值	-5.372
P值	<0.001

分组	例数	表达均值	标准差
非转移组	16	0.9944	0.69116
转移组	14	2.8236	1.14616
t值	-5.372
P值	<0.001

	分组	例数	均值	标准差	t值	P值
侵袭	inhibitor NC	3	303.67	7.51	26.56	P<0.001
侵袭	inhibitor miR-10b	3	103.34	10.70	26.56	P<0.001
迁移	inhibitor NC	3	218.01	12.49	10.40	P<0.001
迁移	inhibitor miR-10b	3	103.00	14.53	10.40	P<0.001

	分组	例数	均值	标准差	t值	P值
侵袭	inhibitor NC	3	303.67	7.51	26.56	P<0.001
侵袭	inhibitor miR-10b	3	103.34	10.70	26.56	P<0.001
迁移	inhibitor NC	3	218.01	12.49	10.40	P<0.001
迁移	inhibitor miR-10b	3	103.00	14.53	10.40	P<0.001

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