Abstract:
Objective To establish a mouse model of colorectal liver metastasis,and to explore the mechanism of CAMP/PKA pathway in colorectal liver metastasis.
Methods To establish a mouse model of colorectal liver metastasis by embedding subcutaneous tumor in the appendix, and randomly divided into experimental group and control group. The mice of experimental group were injected CAMP analogs for 7 days, the mice were sacrificed in 7 days, 14 days, and 28 days after injection. The expression of CAMP, PKA and VEGF in two groups were detected by immunohistochemical method. The expression of MMP2 and E-cadherin in normal tissue and cancer tissue were detected by quantitative PCR.
Results It was succeed to establish a mouse model of colorectal liver metastasis. Comparing to control group, the number of liver metastasis and volume of primary tumor were small in experimental group. The expression of VEGF in the two groups was higher than that in the primary tumor. The expression of E-cadherin in the cancer tissues was lower than that in the normal tissues, and MMP2 was higher than that in the normal tissues. In the experimental group, the expression of CAMP、PKA、VEGF、MMP2 were gradually decreased, while the expression of E-cadherin was increased gradually.
Conclusion CAMP/PKA promoted colorectal liver metastasis by regulating the expression of VEGF, E-cadherin and MMP2.
Key words:
Colorectal neoplasms,
Neovascularization, pathologic,
CAMP/PKA,
Epithelial mesenchymal transition
Jing Qian, Xuetong Jiang, Chuanqi Xu, Daorong Wang. The effect and mechanism of CAMP/PKA pathway in colorectal liver metastases[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2016, 05(03): 244-248.