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中华结直肠疾病电子杂志 ›› 2021, Vol. 10 ›› Issue (02) : 172 -176. doi: 10.3877/cma.j.issn.2095-3224.2021.02.010

所属专题: 文献

论著

血浆外泌体HSP90α对预测结直肠癌肝转移的价值探索
周嘉慧1, 陈思彤1, 刘海洲1, 韦文娥1, 张力图1,()   
  1. 1. 530021 南宁,广西医科大学附属肿瘤医院实验研究部
  • 收稿日期:2020-09-30 出版日期:2021-04-25
  • 通信作者: 张力图
  • 基金资助:
    国家自然基金地区项目(81760535); 广西自然基金面上项目(2017GXNSFAA198155)

The value of plasma exosome HSP90α in predicting colorectal cancer liver metastasis

Jiahui Zhou1, Sitong Chen1, Haizhou Liu1, Wen'e Wei1, Litu Zhang1,()   

  1. 1. Department of Research, Guangxi Medical University Tumor Hospital, Nanning 530021, China
  • Received:2020-09-30 Published:2021-04-25
  • Corresponding author: Litu Zhang
引用本文:

周嘉慧, 陈思彤, 刘海洲, 韦文娥, 张力图. 血浆外泌体HSP90α对预测结直肠癌肝转移的价值探索[J/OL]. 中华结直肠疾病电子杂志, 2021, 10(02): 172-176.

Jiahui Zhou, Sitong Chen, Haizhou Liu, Wen'e Wei, Litu Zhang. The value of plasma exosome HSP90α in predicting colorectal cancer liver metastasis[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2021, 10(02): 172-176.

目的

探讨结直肠癌患者血浆外泌体HSP90α与结直肠癌肝转移的关系。

方法

应用超速离心法提取27例I~III期原发性结直肠癌患者和27例结直肠癌肝转移患者的血浆外泌体,并应用ELISA法检测其HSP90α表达,并与相同患者血浆游离HSP90α比较。

结果

应用超速离心法能有效提取血浆外泌体,经验证符合外泌体相关特征。结直肠癌患者血浆外泌体HSP90α表达水平与患者肿瘤部位有关(H=6.426,P<0.05)。结直肠癌肝转移患者血浆外泌体HSP90α的表达水平高于原发性结直肠癌患者(Z=2.898,P<0.05),与血浆游离HSP90α表达趋势相同。多因素logistics回归分析显示血浆外泌体HSP90α、血浆游离HSP90α、CEA是诊断结直肠癌肝转移的独立危险因素(P<0.05)。血浆外泌体HSP90α联合CEA具有最高的诊断效能。

结论

血浆外泌体HSP90α可能与结直肠癌肝转移生物学功能相关,可以作为传统标志物的有效补充。

Objective

To explore the relationship between plasma exosome HSP90α and colorectal cancer liver metastasis in patients with colorectal cancer.

Methods

The differential expression levels of plasma free HSP90α and exosome HSP90α extracted by ultracentrifugation method were detected by ELISA in 27 patients with stage I-III primary colorectal cancer (pCRC) and 27 patients with colorectal cancer liver metastasis (CRLM).

Results

Ultracentrifugation method can effectively extract plasma exosomes, which have been verified to meet the relevant characteristics of exosomes. The expression level of plasma exosome HSP90α in CRC patients is related to the degree of tumor site (H=6.426, P<0.05). The expression levels of plasma exosome HSP90α in CRLM group were significantly higher than those in pCRC group (Z=2.898, P<0.05), which is consistent with the expression trend of plasma free HSP90α. Multivariate logistics regression analysis showed that plasma exosomes HSP90α, plasma free HSP90α, and CEA were independent risk factors for the diagnosis of colorectal cancer and liver metastasis (P<0.05). And plasma exosome HSP90α combined with CEA has the highest diagnostic efficiency.

Conclusion

Exosome HSP90α may be related to the biological function of colorectal cancer liver metastasis, and can be used as an effective supplement to traditional markers.

图1 外泌体的鉴定。1A:外泌体在电镜下的表现形态(100 nm大小比例尺);1B外泌体表面标记蛋白;1C:ZETAVIEW分析外泌体粒径
表1 结直肠癌患者血浆游离HSP90α和血浆外泌体HSP90α表达水平与临床病理特征的关系
表2 结直肠癌肝转移风险因素的Logistic回归分析
表3 各指标及联合诊断对结直肠癌肝转移诊断的ROC曲线分析结果
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