切换至 "中华医学电子期刊资源库"

中华结直肠疾病电子杂志 ›› 2020, Vol. 09 ›› Issue (02) : 150 -156. doi: 10.3877/cma.j.issn.2095-3224.2020.02.008

所属专题: 文献

论著

利用高通量测序技术探究肠球菌属在Ⅲ期与Ⅳ期结肠癌患者间的丰度差异
王玉柳明1, 张巍远1, 张宇坤1, 胡汉卿1, 黄睿1, 汤庆超1, 陈瑛罡1, 王贵玉1,()   
  1. 1. 150081 哈尔滨医科大学附属第二医院结直肠肿瘤外科
  • 收稿日期:2019-10-10 出版日期:2020-04-25
  • 通信作者: 王贵玉
  • 基金资助:
    国家自然科学基金面上项目(No.81872034); 吴阶平医学基金会(No.320.2710.1849); 中国博士后科学基金(No.2015M581478); 黑龙江省自然科学基金面上项目(No.H2017016)

Exploring the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients based on high-throughput sequencing

Yuliuming Wang1, Weiyuan Zhang1, Yukun Zhang1, Hanqing Hu1, Rui Huang1, Qingchao Tang1, Yinggang Chen1, Guiyu Wang1,()   

  1. 1. Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China
  • Received:2019-10-10 Published:2020-04-25
  • Corresponding author: Guiyu Wang
  • About author:
    Corresponding author: Wang Guiyu, Email:
引用本文:

王玉柳明, 张巍远, 张宇坤, 胡汉卿, 黄睿, 汤庆超, 陈瑛罡, 王贵玉. 利用高通量测序技术探究肠球菌属在Ⅲ期与Ⅳ期结肠癌患者间的丰度差异[J]. 中华结直肠疾病电子杂志, 2020, 09(02): 150-156.

Yuliuming Wang, Weiyuan Zhang, Yukun Zhang, Hanqing Hu, Rui Huang, Qingchao Tang, Yinggang Chen, Guiyu Wang. Exploring the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients based on high-throughput sequencing[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2020, 09(02): 150-156.

目的

利用高通量测序技术探究Ⅲ期与Ⅳ期结肠癌患者的肠球菌属丰度差异。

方法

收集符合标准的结肠癌患者粪便样本,经过基线资料的倾向值匹配后,纳入Ⅲ期组、Ⅳ期组样本各9例。随后进行DNA提取、高通量测序和生物信息学分析。

结果

Alpha多样性分析提示两组在物种多样性和丰富度上差异均有统计学意义(P<0.05)。组间差异性检验提示组间差异大于组内差异,分组正确。从门水平看,两组丰富度差异显著的是疣微菌门。从属水平看,肠球菌属在两组间丰富度差异有统计学意义(P<0.05),且Ⅲ期组肠球菌属丰度更高。

结论

Ⅲ期与Ⅳ期结肠癌患者有着显著不同的菌群特征。肠球菌属丰度的改变可能影响结肠癌疾病的进展。

Objective

To explore the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients.

Methods

After propensity-score matching of baseline characteristics, nine patients of each group who met the inclusion criteria were finally included in this study. After collecting their fecal samples and extracting total DNA, we put forward high-throughput sequencing and bioinformatic analysis.

Results

Alpha diversity analysis showed that two groups were significantly different in diversity and abundance of OTU (P<0.05). The difference between two groups was greater than difference within the group according to result of ANOSIM. The differential microbiota was classified as Verrucomicrobia on phylum level. On genus level, the abundance of Enterococcus was significantly different between two groups (P<0.05) and the abundance of Enterococcus in stage Ⅲ group was much higher.

Conclusions

The characteristics of gut microbiota of stage Ⅲ colon cancer patients were significantly different from stage Ⅳ colon cancer patients. The altered abundance of Enterococcus might affect the tumor progression.

表1 倾向值匹配结果[例(%)]
表2 匹配后患者病理信息表[例(%)]
图1 Shannon-wiener曲线图
图2 属水平群落Heatmap图
表3 Alpha多样性指数表
图3 基于OTU水平的主坐标分析(3A:unweighted_unifric算法,R=0.570,P=0.001;3B:weighted_unifric算法,R=0.236,P=0.018)
图4 门水平两组肠道菌群差异图
图5 属水平两组肠道菌群差异图
图6 LDA判别柱形图
[1]
赫捷, 陈万青. 2017中国肿瘤登记年报[M].北京:人民卫生出版社, 2018.
[2]
Yu T, Guo F, Yu Y, et al. Fusobacterium nucleatum promotes chemoresistance to colorectal cancer by modulating autophagy [J]. Cell, 2017, 170(3): 548-563.
[3]
Tsoi H, Chu ESH, Zhang X, et al. Peptostreptococcus anaerobius induces intracellular cholesterol biosynthesis in colon cells to induce proliferation and causes dysplasia in mice [J]. Gastroenterology, 2017, 152(6): 1419-1433.
[4]
Jin Y, Tang S, Li W, et al. Hemolytic E.coli promotes colonic tumorigenesis in females [J]. Cancer Res, 2016, 76(10): 2891-2900.
[5]
Kostic AD, Chun E, Robertson L, et al. Fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor-immune microenvironment [J]. Cell Host Microbe, 2013, 14(2): 207-215.
[6]
Kawano A, Ishikawa H, Mutoh M, et al. Higher enterococcus counts indicate a lower risk of colorectal adenomas: a prospective cohort study [J]. Oncotarget, 2018, 9(30): 21459-21467.
[7]
Huycke MM, Abrams V, Moore DR. Enterococcus faecalis produces extracellular superoxide and hydrogen peroxide that damages colonic epithelial cell DNA [J]. Carcinogenesis, 2002, 23(3): 529-536.
[8]
Nakatsu G, Li X, Zhou H, et al. Gut mucosal microbiome across stages of colorectal carcinogenesis [J]. Nat Commun, 2015, 6: 8727.
[9]
Al-Homoud S, Purkayastha S, Aziz O, et al. Evaluating operative risk in colorectal cancer surgery: ASA and POSSUM-based predictive models [J]. Surg Oncol, 2004, 13(2-3): 83-92.
[10]
陈文杰, 苏献豪, 王跃迁, 等. 姜黄素干预小鼠肠道菌群并抑制结直肠癌变的实验研究 [J/CD]. 中华结直肠疾病电子杂志, 2018, 7(6): 538-545.
[11]
Yan X, Liu L, Li H, et al. Clinical significance of Fusobacterium nucleatum, epithelial-mesenchymal transition, and cancer stem cell markers in stage Ⅲ/Ⅳ colorectal cancer patients [J]. Onco Targets Ther, 2017, 10: 5031-5046.
[12]
Balamurugan R, Rajendiran E, George S, et al. Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer [J]. J Gastroenterol Hepatol, 2008, 23(8): 1298-1303.
[13]
De Almeida CV, Lulli M, di Pilato V, et al. Differential responses of colorectal cancer cell lines to enterococcus faecalis' strains isolated from healthy donors and colorectal cancer patients [J]. J Clin Med, 2019, 8(3): E388.
[14]
Wong SH, Kwong TNY, Chow TC, et al. Quantitation of faecal Fusobacterium improves faecal immunochemical test in detecting advanced colorectal neoplasia [J]. Gut, 2017, 66(8): 1441-1448.
[15]
Fu Y, Wang L, Xie C, et al. Comparison of non-invasive biomarkers faecal BAFF, calprotectin and FOBT in discriminating IBS from IBD and evaluation of intestinal inflammation [J]. Sci Rep, 2017, 7(1): 2669.
[16]
Todorov K, Wilson C, Sharplin G, et al. Faecal occult blood testing (FOBT)- based colorectal cancer screening trends and predictors of non-use: findings from the South Australian setting and implications for increasing FOBT uptake [J]. Aust Health Rev, 2018, 42(1): 45-52.
[1] 燕速, 霍博文, 徐惠宁. 4K荧光腹腔镜扩大右半结肠CME+D3根治术及No.206、No.204组淋巴结清扫术[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 14-14.
[2] 姚宏伟, 魏鹏宇, 高加勒, 张忠涛. 不断提高腹腔镜右半结肠癌D3根治术的规范化[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 1-4.
[3] 杜晓辉, 崔建新. 腹腔镜右半结肠癌D3根治术淋巴结清扫范围与策略[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 5-8.
[4] 周岩冰, 刘晓东. 腹腔镜右半结肠癌D3根治术消化道吻合重建方式的选择[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 9-13.
[5] 徐伯麒, 陶亮, 章帆, 毛忠琦. 结肠癌患者淋巴结转移预测模型的建立[J]. 中华普外科手术学杂志(电子版), 2023, 17(04): 393-397.
[6] 彭雨诗, 苗芸, 严紫嫣. 宏基因组高通量测序诊断肾移植术后华支睾吸虫感染一例[J]. 中华移植杂志(电子版), 2023, 17(05): 297-299.
[7] 蓝冰, 王怀明, 王辉, 马波. 局部晚期结肠癌膀胱浸润的研究进展[J]. 中华结直肠疾病电子杂志, 2023, 12(06): 505-511.
[8] 唐新, 刁德昌, 廖伟林, 林佳鑫, 汪佳豪, 李文娟, 谢嘉欣, 敖琳, 李洪明, 易小江, 卢新泉, 冯晓创. 保留神经的鞘外游离技术在腹腔镜右半结肠癌D3根治术中的近远期疗效分析:基于倾向性评分匹配的回顾性队列研究[J]. 中华结直肠疾病电子杂志, 2023, 12(05): 372-380.
[9] 陈润芝, 杨东梅, 徐慧婷. 信迪利单抗联合索凡替尼后线治疗MSS型BRAF突变的转移性结肠癌:个案报道并文献复习[J]. 中华结直肠疾病电子杂志, 2023, 12(05): 431-435.
[10] 侯文运, 刘恒昌, 窦利州, 陈海鹏, 郑朝旭, 王贵齐, 王锡山. 腹部无辅助切口内镜引导下取标本的腹腔镜辅助右半结肠癌根治术(保留回盲部)(附视频)[J]. 中华结直肠疾病电子杂志, 2023, 12(05): 436-440.
[11] 常远, 白杨, 王文秀, 孙振强, 周全博, 崔雯铭, 王玉行, 袁维堂, 胡军红. 电子结肠镜辅助经结肠取标本手术在结肠肿瘤中的临床应用分析[J]. 中华结直肠疾病电子杂志, 2023, 12(03): 196-199.
[12] 邓振汝, 林树文, 吕泽坚, 郑佳彬, 廖乾超, 冯伙伦, 吴德庆, 王俊江, 李勇. 横结肠切除与扩大结肠切除治疗横结肠癌疗效比较[J]. 中华结直肠疾病电子杂志, 2023, 12(03): 214-220.
[13] 路继永, 王帅, 张成仁, 吴德望, 杨熊飞. 腹腔镜右半结肠切除应用现状[J]. 中华结直肠疾病电子杂志, 2023, 12(03): 221-227.
[14] 李峻峰, 李军, 孙勤丰, 孙建光, 孔祥兴. 九例结肠髓样癌的临床病理特征分析[J]. 中华结直肠疾病电子杂志, 2023, 12(03): 248-252.
[15] 徐正, 苏昊, 包满都拉, 罗寿, 张岳阳, 关旭, 张明光, 赵志勋, 梁建伟, 刘正, 刘骞, 王锡山, 周海涛. 经末段回肠系膜后隧道完成重叠式三角吻合的结肠脾曲癌完全腹腔镜左半结肠切除术23例临床分析[J]. 中华结直肠疾病电子杂志, 2023, 12(03): 253-259.
阅读次数
全文


摘要