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中华结直肠疾病电子杂志 ›› 2020, Vol. 09 ›› Issue (02) : 150 -156. doi: 10.3877/cma.j.issn.2095-3224.2020.02.008

所属专题: 文献

论著

利用高通量测序技术探究肠球菌属在Ⅲ期与Ⅳ期结肠癌患者间的丰度差异
王玉柳明1, 张巍远1, 张宇坤1, 胡汉卿1, 黄睿1, 汤庆超1, 陈瑛罡1, 王贵玉1,()   
  1. 1. 150081 哈尔滨医科大学附属第二医院结直肠肿瘤外科
  • 收稿日期:2019-10-10 出版日期:2020-04-25
  • 通信作者: 王贵玉
  • 基金资助:
    国家自然科学基金面上项目(No.81872034); 吴阶平医学基金会(No.320.2710.1849); 中国博士后科学基金(No.2015M581478); 黑龙江省自然科学基金面上项目(No.H2017016)

Exploring the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients based on high-throughput sequencing

Yuliuming Wang1, Weiyuan Zhang1, Yukun Zhang1, Hanqing Hu1, Rui Huang1, Qingchao Tang1, Yinggang Chen1, Guiyu Wang1,()   

  1. 1. Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China
  • Received:2019-10-10 Published:2020-04-25
  • Corresponding author: Guiyu Wang
  • About author:
    Corresponding author: Wang Guiyu, Email:
引用本文:

王玉柳明, 张巍远, 张宇坤, 胡汉卿, 黄睿, 汤庆超, 陈瑛罡, 王贵玉. 利用高通量测序技术探究肠球菌属在Ⅲ期与Ⅳ期结肠癌患者间的丰度差异[J/OL]. 中华结直肠疾病电子杂志, 2020, 09(02): 150-156.

Yuliuming Wang, Weiyuan Zhang, Yukun Zhang, Hanqing Hu, Rui Huang, Qingchao Tang, Yinggang Chen, Guiyu Wang. Exploring the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients based on high-throughput sequencing[J/OL]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2020, 09(02): 150-156.

目的

利用高通量测序技术探究Ⅲ期与Ⅳ期结肠癌患者的肠球菌属丰度差异。

方法

收集符合标准的结肠癌患者粪便样本,经过基线资料的倾向值匹配后,纳入Ⅲ期组、Ⅳ期组样本各9例。随后进行DNA提取、高通量测序和生物信息学分析。

结果

Alpha多样性分析提示两组在物种多样性和丰富度上差异均有统计学意义(P<0.05)。组间差异性检验提示组间差异大于组内差异,分组正确。从门水平看,两组丰富度差异显著的是疣微菌门。从属水平看,肠球菌属在两组间丰富度差异有统计学意义(P<0.05),且Ⅲ期组肠球菌属丰度更高。

结论

Ⅲ期与Ⅳ期结肠癌患者有着显著不同的菌群特征。肠球菌属丰度的改变可能影响结肠癌疾病的进展。

Objective

To explore the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients.

Methods

After propensity-score matching of baseline characteristics, nine patients of each group who met the inclusion criteria were finally included in this study. After collecting their fecal samples and extracting total DNA, we put forward high-throughput sequencing and bioinformatic analysis.

Results

Alpha diversity analysis showed that two groups were significantly different in diversity and abundance of OTU (P<0.05). The difference between two groups was greater than difference within the group according to result of ANOSIM. The differential microbiota was classified as Verrucomicrobia on phylum level. On genus level, the abundance of Enterococcus was significantly different between two groups (P<0.05) and the abundance of Enterococcus in stage Ⅲ group was much higher.

Conclusions

The characteristics of gut microbiota of stage Ⅲ colon cancer patients were significantly different from stage Ⅳ colon cancer patients. The altered abundance of Enterococcus might affect the tumor progression.

表1 倾向值匹配结果[例(%)]
表2 匹配后患者病理信息表[例(%)]
图1 Shannon-wiener曲线图
图2 属水平群落Heatmap图
表3 Alpha多样性指数表
图3 基于OTU水平的主坐标分析(3A:unweighted_unifric算法,R=0.570,P=0.001;3B:weighted_unifric算法,R=0.236,P=0.018)
图4 门水平两组肠道菌群差异图
图5 属水平两组肠道菌群差异图
图6 LDA判别柱形图
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