细胞自噬与结肠癌Lovo细胞迁移和侵袭的相关性研究

doi:10.3877/cma.j.issn.2095-3224.2013.02.04

中华结直肠疾病电子杂志 ›› 2013, Vol. 02 ›› Issue (02) : 63 -67. doi: 10.3877/cma.j.issn.2095-3224.2013.02.04

所属专题：文献；

论著

细胞自噬与结肠癌Lovo细胞迁移和侵袭的相关性研究

王天宝¹^,^†(

), 石汉平¹, 韩方海¹, 董文广¹

^1. 510080　广州，中山大学附属第一医院外科

收稿日期:2013-01-06 出版日期:2013-04-25
通信作者: 王天宝
基金资助:
广东省科技计划资助项目(2012B060900100)

The relationship of cell autophagy and Lovo cell migration and invasion

Tian-bao WANG¹^,^†(), Han-ping SHI¹, Fang-hai HAN¹, Wen-guang DONG¹

^1. Department of Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China

Received:2013-01-06 Published:2013-04-25
Corresponding author: Tian-bao WANG
About author:
Corresponding author: WANG Tian-bao, Email: wangtianbao1@163.com

全文 ( ) 下载PDF ( ) 导出引用

目的

探讨不同自噬状态对结直肠癌Lovo细胞迁移和侵袭能力的影响。

方法

将Lovo细胞分为自噬增强组、正常细胞组和3-甲基嘌呤自噬抑制组。用激光共聚焦显微镜观察绿色荧光颗粒，用Q-PCR检测Beclin1 mRNA表达水平，透射电镜观察自噬溶酶体，Transwell实验评价Lovo细胞迁移与侵袭能力。

结果

绿色荧光颗粒数以自噬增强组最多，其次为正常细胞组，而自噬抑制组最少。Beclin1 mRNA表达量自噬增强组为(1.23±0.12)个，正常细胞组为(1±0.13)个，自噬抑制组为(0.98±0.1)个。自噬增强组可见大量的自噬溶酶体，明显多于正常细胞组和自噬抑制组。迁移实验细胞计数：自噬增强组高于正常细胞组(138.0±16.7与90.7±12.9，P＝0.026)和自噬抑制组(138.0±16.7与92.7±26.7，P＝0.030)。侵袭实验细胞计数：自噬增强组高于正常细胞组(147.0±13.0与99.0±20.5，P＝0.028)和自噬抑制组(147.0±13.0与95.7±25.6，P＝0.021)。

结论

结肠癌Lovo细胞自噬增强促进肿瘤细胞迁移和浸润，可能是局部浸润和远处转移的机制之一。

关键词: 自噬, 细胞运动, 淋巴细胞，肿瘤浸润, Lovo细胞

Objective

To investigate the influence of autophagy on migration and invasion in colon cancer cells.

Methods

The Lovo cells of colon cancer were classified as autophagy-enhanced group, normal cell group, and 3-Methyladenine autophagy-inhibited group.Laser canning confocal microscope was used to identify the green fluorescence particles.Beclin1 mRNA expressive level was measured with Q-PCR.Transmission electron microscope was employed to observe the autolysosome.Transwell chamber was applied to evaluate the migration and invasion of Lovo cell.

Results

There were more green fluorescence particles in the autophagy-enhanced group than the normal cell group and the autophagy-inhibited group.The Beclin 1 mRNA expressive level was 1.23±0.12 in the autophagy-enhanced group, while 1±0.13 in the normal cell group and 0.98±0.1 in the autophagy-inhibited group.There were the most autolysosomes in autophagy-enhanced group of all groups.The migration experiment showed the mean number of Lovo cell in the autophagy-enhanced group(138.0±16.7)were more than the normal Lovo cell group(90.7±12.9, P=0.026)and the autophagy-inhibited group(92.7±26.7, P=0.030). The invasion experiment found the mean number of Lovo cell in autophagy-enhanced group(147.0±13.0)were also more than the normal Lovo cell group(99.0±20.5, P=0.028)and the autophagy-inhibited group(95.7±25.6, P=0.021).

Conclusions

Autophagy enhancement could promote the migrating and invasive ability of Lovo cell.And that might be an important mechanism of invasion and metastasis in malignant tumors.

Key words: Autophagy, Cell movement, Lymphocytes, tumor-infiltrating, Lovo cell

图1 激光共聚焦显微镜镜下观察自噬增强组、正常细胞组和自噬抑制组Lovo细胞内荧光颗粒图像

图2 透视电镜下观察自噬增强组细胞、正常细胞组和3-MA自噬抑制组Lovo细胞的自噬溶酶体图像

[1]	Parsons HM，Tuttle TM，Kuntz KM，et al.Association between lymph node evaluation for colon cancer and node positivity over the past 20 years.JAMA，2011,306(10):1089-1097.
[2]	Rice J．Metastasis：The rude awakening.Nature，2012,485(7400):55-57.
[3]	Behrends C，Sowa ME，Gygi SP，et al.Network organization of the human autophagy system.Nature，2010,466(7302):68-76.
[4]	Buchser WJ，Laskow TC，Pavlik PJ，et al.Cell-mediated autophagy promotes cancer cell survival.Cancer Res，2012,72(12):2970-2979.
[5]	Yoshioka A，Miyata H，Doki Y，et al.LC3，an autophagosome marker，is highly expressed in gastrointestinal cancers.Int J Oncol，2008,33(3):461-468.
[6]	Habeeb BS，Kitayama J，Nagawa H．Adiponectin supports cell survival in glucose deprivation through enhancement of autophagic response in colorectal cancer cells.Cancer Sci，2011,102(5):999-1006.
[7]	Sancak Y，Bar-Peled L，Zoncu R，et al.Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids.Cell，2010,141(2):290-303.
[8]	Groulx JF，Khalfaoui T，Benoit YD，et al.Autophagy is active in normal colon mucosa.Autophagy，2012,8(6):893-902.
[9]	Giatromanolaki A，Koukourakis MI，Harris AL，et al.Prognostic relevance of light chain 3(LC3A)autophagy patterns in colorectal adenocarcinomas.J Clin Pathol，2010,63(10):867-872.
[10]	Chen F，Wang CC，Kim E，et al.Hyperthermia in combination with oxidative stress induces autophagic cell death in HT-29 colon cancer cells.Cell Biol Int，2008,32(7):715-723.
[11]	Scherz-Shouval R，Weidberg H，Gonen C，et al.p53-dependent regulation of autophagy protein LC3 supports cancer cell survival under prolonged starvation.Proc Natl Acad Sci USA，2010,107(43):18511-1856.
[12]	Li J，Hou N，Faried A，et al.Inhibition of autophagy augments 5-fluorouracil chemotherapy in human colon cancer in vitro and in vivo model.Eur J Cancer，2010,46(10):1900-1909.
[13]	Sasaki K，Tsuno NH，Sunami E，et al.Resistance of colon cancer to 5-fluorouracil may be overcome by combination with chloroquine，an in vivo study.Anticancer Drugs，2012,23(7):675-782.
[14]	王天宝，石汉平，韩方海，等．SDF-1/CXCR4系统和结直肠癌转移的相关性研究进展．中华结直肠疾病电子杂志，2012,1(1):31-33.
[15]	Teicher BA，Fricker SP.CXCL12(SDF-1)/CXCR4 pathway in cancer.Clin Cancer Res，2010,16(11):2927-2931.

[1]	张刚, 秦勇, 黄超, 薛震, 吕松岑. 基于骨关节炎软骨细胞表型转化的新兴治疗靶点[J/OL]. 中华关节外科杂志(电子版), 2024, 18(03): 352-362.
[2]	王帆, 余辉, 谢佳乐, 许焕焕, 马瑞, 依日夏提·艾海提, 许珂, 许鹏. 成纤维样滑膜细胞在类风湿关节炎发病机制中的作用[J/OL]. 中华关节外科杂志(电子版), 2024, 18(02): 225-230.
[3]	吴杰, 周志强, 符菁, 李喜功, 张钦. 吸入性氢气对大鼠脊髓损伤后自噬及神经功能的影响[J/OL]. 中华危重症医学杂志(电子版), 2024, 17(05): 363-371.
[4]	许彬, 王丽, 陈瑞, 沈奕, 陆件. 瞬时受体电位粘脂素1介导细胞自噬在远端缺血后处理保护大鼠脑缺血-再灌注损伤中的作用研究[J/OL]. 中华危重症医学杂志(电子版), 2024, 17(03): 180-187.
[5]	孔莹莹, 谢璐涛, 卢晓驰, 徐杰丰, 周光居, 张茂. 丁酸钠对猪心脏骤停复苏后心脑损伤的保护作用及机制研究[J/OL]. 中华危重症医学杂志(电子版), 2023, 16(05): 355-362.
[6]	韩春颖, 王婷婷, 李艳艳, 朴金霞. 子宫内膜癌患者淋巴管间隙浸润预测因素研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(04): 403-409.
[7]	李争光, 宰爽嘉, 吴火峰, 孙华, 张永博, 陈浏阳, 戴睿, 张亮. 昼夜节律相关因子在椎间盘退行性变发病机制中作用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(05): 457-461.
[8]	周子慧, 李恭驰, 李炳辉, 王知, 刘慧真, 王卉, 邹利军. 细胞自噬在创面愈合中作用的研究进展[J/OL]. 中华损伤与修复杂志(电子版), 2023, 18(06): 542-546.
[9]	廖泽楷, 梁爱琳, 龚启梅. 根尖周病中程序性细胞死亡的研究进展[J/OL]. 中华口腔医学研究杂志(电子版), 2024, 18(03): 150-155.
[10]	史清泉, 苗彬, 王烁, 陶琳, 沈晨. miR-181a-5p 靶向ATG5 抑制雨蛙素诱导的大鼠胰腺腺泡细胞AR42J自噬的机制研究[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 524-530.
[11]	王小红, 钱晶, 翁文俊, 周国雄, 朱顺星, 祁小鸣, 刘春, 王萍, 沈伟, 程睿智, 秦璟灏. 巯基丙酮酸硫基转移酶调控核因子κB信号介导自噬对重症急性胰腺炎大鼠的影响及机制[J/OL]. 中华消化病与影像杂志(电子版), 2023, 13(06): 422-426.
[12]	于伟伟, 张国高, 吴军, 胡俊, 黄一宁, 徐晶. 线粒体相关内质网膜相关线粒体功能障碍在阿尔茨海默病中的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(02): 223-230.
[13]	包文华, 塔拉. 自噬及内质网应激在卡非佐米对MCF-7细胞的影响及作用机制[J/OL]. 中华临床医师杂志(电子版), 2023, 17(11): 1181-1191.
[14]	刘霖, 张文欢, 宋雅茹, 姜云璐. Apelin-13 在阿尔茨海默病中的神经保护作用机制研究进展[J/OL]. 中华诊断学电子杂志, 2024, 12(04): 276-280.
[15]	邱甜, 杨苗娟, 胡波, 郭毅, 何奕涛. 亚低温治疗脑梗死机制的研究进展[J/OL]. 中华脑血管病杂志(电子版), 2023, 17(05): 518-521.

阅读次数

全文

摘要

选择文件类型/文献管理软件名称

选择包含的内容

The relationship of cell autophagy and Lovo cell migration and invasion

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

The relationship of cell autophagy and Lovo cell migration and invasion