Effect of RNA-binding protein with multiple splicing on colitis associated colon cancer

doi:10.3877/cma.j.issn.2095-3224.2021.03.007

Abstract

Abstract:

Objective

To investigate the expression of RNA alternative splicing protein RBPMS in colitis associated colon cancer and its effect on the proliferation of colon cancer cells.

Methods

Colitis-associated colon cancer was induced by azoxymethane (AOM) and dextran sulfate sodium (DSS) in C57BL/6 mice. Mice of four different stages during the development of colon cancer were obtained (inflammatory recovery stage, mild dysplasia, Adenoma, Adenocarcinoma), and a control group was established without any treatment. RNA sequencing was performed in different stages of colon tissue. HCT116 cells were transfected with specific small interfering RNA (siRNA) using Lipofectamine^TM 2 000. The total protein was extracted 72 hours later, and the knock-down efficiency was measured by Western blot. The effect of RBPMS knockdown on cell proliferation and clone formation ability was examined by MTT and clone-forming assay. Cell cycle were measured by Flow cytometry.

Results

The RNA sequencing results showed that the expression level of RBPMS mRNA increased with the development of colitis associated colon cancer. The results showed that knocking down RBPMS significantly inhibited the proliferation and clone forming ability of tumor cells by inducing G0/G1 arrest.

Conclusion

RBPMS is overexpressed in colitis associated colon cancer tissue, and RBPMS knock down can inhibit the proliferation of HCT116 cells.

Key words: RBPMS, Proliferation, Colitis associated Colon cancer

Xinhua Zhao, Yiming Ma, Longmei He, Hongying Wang. Effect of RNA-binding protein with multiple splicing on colitis associated colon cancer[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2021, 10(03): 260-265.

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Figures/Tables 6

References 23

1	Liu Y, Liu X, Lin C, et al. Noncoding RNAs regulate alternative splicing in Cancer[J]. J Exp Clin Cancer Res, 2021, 40(1): 11.
2	Liu Z, Rabadan R. Computing the role of alternative splicing in cancer[J]. Trends Cancer, 2021, 7(4): 347-358.
3	Wu S, Wang J, Zhu X, et al. The functional impact of alternative splicing on the survival prognosis of triple-negative breast cancer[J]. Front Genet, 2020, 11: 604262.
4	Shimamoto A, Kitao S, Ichikawa K, et al. A unique human gene that spans over 230 kb in the human chromosome 8p11-12 and codes multiple family proteins sharing RNA-binding motifs[J]. Proc Natl Acad Sci USA, 1996, 93(20): 10913-10917.
5	Yabana T, Hooper ML, Farrell SR, et al. GCaMP3 expressing cells in the ganglion cell layer of Thy1-GCaMP3 transgenic mice before and after optic nerve injury[J]. Exp Eye Res, 2021, 202: 108297.
6	Akerberg AA, Burns CE, Burns CG. Exploring the activities of RBPMS proteins in myocardial biology[J]. Pediatr Cardiol, 2019, 40(7): 1410-1418.
7	Fu J, Cheng L, Wang Y, et al. The RNA-binding protein RBPMS1 represses AP-1 signaling and regulates breast cancer cell proliferation and migration[J]. Biochim Biophys Acta, 2015, 1853(1): 1-13.
8	Parang B, Barrett CW, Williams CS. AOM/DSS model of colitis-associated cancer[J]. Methods Mol Biol, 2016, 1422: 297-307.
9	Liu X, Wei W, Li X, et al. BMI1 and MEL18 promote colitis-associated cancer in mice via REG3B and STAT3[J]. Gastroenterology, 2017, 153(6): 1607-1620.
10	De Robertis M, Massi E, Poeta ML, et al. The AOM/DSS murine model for the study of colon carcinogenesis: From pathways to diagnosis and therapy studies[J]. J Carcinog, 2011, 10: 9.
11	Oltean S, Bates DO. Hallmarks of alternative splicing in cancer[J]. Oncogene, 2014, 33(46): 5311-5318.
12	Soo V, Swadling JB, Faure AJ, et al. Fitness landscape of a dynamic RNA structure[J]. PLoS Genet, 2021, 17(2): e1009353.
13	Black DL. Mechanisms of alternative pre-messenger RNA splicing[J]. Annu Rev Biochem, 2003, 72: 291-336.
14	Wang Y, Chen D, Qian H, et al. The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression[J]. Cancer Cell, 2014, 26(3): 374-389.
15	Stevens M, Oltean S. Modulation of the apoptosis gene Bcl-x function through alternative splicing[J]. Front Genet, 2019, 10: 804.
16	Weldon C, Dacanay JG, Gokhale V, et al. Specific G-quadruplex ligands modulate the alternative splicing of Bcl-X[J]. Nucleic Acids Res, 2018, 46(2): 886-896.
17	Francies FZ, Bassa S, Chatziioannou A, et al. Splicing genomics events in cervical cancer: Insights for phenotypic stratification and biomarker potency[J]. Genes (Basel), 2021, 12(2).
18	Jiang J, Chen X, Liu H, et al. Erratum: Polypyrimidine Tract-Binding Protein 1 promotes proliferation, migration and invasion in clear-cell renal cell carcinoma by regulating alternative splicing of PKM[J]. Am J Cancer Res, 2021, 11(1): 297-300.
19	Sciarrillo R, Wojtuszkiewicz A, Assaraf YG, et al. The role of alternative splicing in cancer: From oncogenesis to drug resistance[J]. Drug Resist Updat, 2020, 53: 100728.
20	Rastgoo N, Pourabdollah M, Abdi J, et al. Dysregulation of EZH2/miR-138 axis contributes to drug resistance in multiple myeloma by downregulating RBPMS[J]. Leukemia, 2018, 32(11): 2471-2482.
21	Baez-Vega PM, Echevarria VI, Valiyeva F, et al. Targeting miR-21-3p inhibits proliferation and invasion of ovarian cancer cells[J]. Oncotarget, 2016, 7(24): 36321-36337.
22	Baughn LB, Di Liberto M, Niesvizky R, et al. CDK2 phosphorylation of Smad2 disrupts TGF-beta transcriptional regulation in resistant primary bone marrow myeloma cells[J]. J Immunol, 2009, 182(4): 1810-1817.
23	Sun Y, Ding L, Zhang H, et al. Potentiation of Smad-mediated transcriptional activation by the RNA-binding protein RBPMS[J]. Nucleic Acids Res, 2006, 34(21): 6314-6326.

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