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Chinese Journal of Colorectal Diseases(Electronic Edition) ›› 2020, Vol. 09 ›› Issue (02): 150-156. doi: 10.3877/cma.j.issn.2095-3224.2020.02.008

Special Issue:

• Original Article • Previous Articles     Next Articles

Exploring the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients based on high-throughput sequencing

Yuliuming Wang1, Weiyuan Zhang1, Yukun Zhang1, Hanqing Hu1, Rui Huang1, Qingchao Tang1, Yinggang Chen1, Guiyu Wang1,()   

  1. 1. Department of Colorectal Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150081, China
  • Received:2019-10-10 Online:2020-04-25 Published:2020-04-25
  • Contact: Guiyu Wang
  • About author:
    Corresponding author: Wang Guiyu, Email:

Abstract:

Objective

To explore the differential abundance of Enterococcus between stage Ⅲ and stage Ⅳ colon cancer patients.

Methods

After propensity-score matching of baseline characteristics, nine patients of each group who met the inclusion criteria were finally included in this study. After collecting their fecal samples and extracting total DNA, we put forward high-throughput sequencing and bioinformatic analysis.

Results

Alpha diversity analysis showed that two groups were significantly different in diversity and abundance of OTU (P<0.05). The difference between two groups was greater than difference within the group according to result of ANOSIM. The differential microbiota was classified as Verrucomicrobia on phylum level. On genus level, the abundance of Enterococcus was significantly different between two groups (P<0.05) and the abundance of Enterococcus in stage Ⅲ group was much higher.

Conclusions

The characteristics of gut microbiota of stage Ⅲ colon cancer patients were significantly different from stage Ⅳ colon cancer patients. The altered abundance of Enterococcus might affect the tumor progression.

Key words: Colonic neoplasms, Enterococcus, High-throughput sequencing

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