Association of prolactin receptor experssion in human perianal abscess with postoperative prognosis

doi:10.3877/cma.j.issn.2095-3224.2017.06.009

Abstract

Abstract:

Objective

To investigate the association of prolactin receptor (PRLR) expression in human perianal abscess with its prognosis,which may be a new prognosis biomarker of perianal abscess-related.

Methods

Clinical data and resection samples of 60 patients with perianal abscess identified by pathology undergoing resection in our department from August 2015 to August 2016 were collected as well as 8 patients receiving only hemorroidectomy (control group), matching with perianal abscess group in age, gender and body weight. The PRLR expression in perianal abscess tissues and matched nonabscess tissues collected was detected by immunohistochemical staining and Western blotting. The associations of PRLR level with overall prognosis,clinicopathological features were analyzed, univariate and multivariate logistic regression analysis.

Results

Immunohistochemical staining showed that the positive brown granules were mainly distributed on the cell membrane,and also in cytoplasm partially. And the low expression of PRLR protein in perianal abscess group was 81.67% (41/60) and high espression wsa 31.67% (19/60), which was significantly higher than that of control group (P<0.05). The relative gray value of Western blotting in perianal abscess tissues were 0.515±0.067, which were significantly lower than those of matched nonabscess tissues (0.836±0.035 and P<0.05). Patients with low expression of PRLR protein had poor prognosis than those with low expression (68.85% vs. 36.84%, P< 0.05). Univariate and Multivariate logistic regression analysis showed that the complexity of abscess (OR=7.911, 95%CI: 1.115~20.161, P=0.039), the number of surgical incision (OR=3.246, 95%CI: 0.774~5.742, P=0.042) and the expression of PRLR protein (OR=9.099, 95%CI: 1.727~14.426, P=0.016) were independent risk factors for poor prognosis of patients with perianal abscess (all P<0.05).

Conclusions

PRLP expression in perianal abscess patient was low, which may be a valuable in predicting prognosis.

Key words: Anal canal, Prognosis, Prolactin receptor, Perianal abscess

Yong Tao, Guangen Yang, Jianming Qiu, Kan Xu, Dong Wang, Hongtao Wang. Association of prolactin receptor experssion in human perianal abscess with postoperative prognosis[J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2017, 06(06): 488-493.

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References 18

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临床病理特征		例数	PRLR表达		χ²值	P值
临床病理特征		例数	低表达	高表达	χ²值	P值
年龄（岁）					0.960	0.327
	<45	37	27	10
	≥45	23	14	9
性别					0.477	0.490
	男	34	22	12
	女	26	19	7
BMI指数					2.330	0.127
	<24	48	35	13
	≥24	12	6	6
药物治疗史					2.080	0.149
	有	45	33	12
	无	15	8	7
脓肿范围					6.406	0.011
	单象限	21	10	11
	多象限	39	31	8
脓肿位置					0.960	0.327
	前后位	23	14	9
	两侧位	37	27	10
脓肿性质					6.782	0.009
	单纯性	18	8	10
	复杂性	42	33	9

临床病理特征		例数	PRLR表达		χ²值	P值
临床病理特征		例数	低表达	高表达	χ²值	P值
年龄（岁）					0.960	0.327
	<45	37	27	10
	≥45	23	14	9
性别					0.477	0.490
	男	34	22	12
	女	26	19	7
BMI指数					2.330	0.127
	<24	48	35	13
	≥24	12	6	6
药物治疗史					2.080	0.149
	有	45	33	12
	无	15	8	7
脓肿范围					6.406	0.011
	单象限	21	10	11
	多象限	39	31	8
脓肿位置					0.960	0.327
	前后位	23	14	9
	两侧位	37	27	10
脓肿性质					6.782	0.009
	单纯性	18	8	10
	复杂性	42	33	9

临床病理特征		总例数	未愈（n=34）（%）	95%CI值	P值
年龄（岁）				0.805~7.230	0.116
	<45	37	18（48.65）
	≥45	23	16（69.56）
性别				0.292~2.288	0.700
	男	34	20（58.82）
	女	26	14（53.84）
BMI指数				0.449~6.348	0.437
	<24	48	26（54.12）
	≥24	12	8（66.67）
药物治疗史				0.528~5.561	0.369
	无	15	7（46.67）
	有	45	27（60.00）
脓肿范围				1.963~20.625	0.002
	单象限	21	6（28.57）
	多象限	39	28（71.79）
脓肿位置				0.192~1.647	0.294
	前后位	23	15（65.22）
	两侧位	37	19（51.35）
脓肿性质				1.710~19.673	0.005
	单纯性	18	5（27.78）
	复杂性	42	29（69.05）
脓肿切口				0.069~0.802	0.021
	单切口	44	29（65.91）
	多切口	16	5（31.25）
术后抗生素应用				0.067~0.656	0.007
	无	26	20（76.92）
	有	34	14（41.18）
PRLR表达量				2.010~41.796	0.004
	高表达	19	7（36.84）
	低表达	41	27（65.85）

临床病理特征		总例数	未愈（n=34）（%）	95%CI值	P值
年龄（岁）				0.805~7.230	0.116
	<45	37	18（48.65）
	≥45	23	16（69.56）
性别				0.292~2.288	0.700
	男	34	20（58.82）
	女	26	14（53.84）
BMI指数				0.449~6.348	0.437
	<24	48	26（54.12）
	≥24	12	8（66.67）
药物治疗史				0.528~5.561	0.369
	无	15	7（46.67）
	有	45	27（60.00）
脓肿范围				1.963~20.625	0.002
	单象限	21	6（28.57）
	多象限	39	28（71.79）
脓肿位置				0.192~1.647	0.294
	前后位	23	15（65.22）
	两侧位	37	19（51.35）
脓肿性质				1.710~19.673	0.005
	单纯性	18	5（27.78）
	复杂性	42	29（69.05）
脓肿切口				0.069~0.802	0.021
	单切口	44	29（65.91）
	多切口	16	5（31.25）
术后抗生素应用				0.067~0.656	0.007
	无	26	20（76.92）
	有	34	14（41.18）
PRLR表达量				2.010~41.796	0.004
	高表达	19	7（36.84）
	低表达	41	27（65.85）

因素	OR值	95% CI值	P值
脓肿范围（单象限：多象限）	2.101	0.238~18.561	0.504
脓肿性质（单纯性：复杂性）	7.911	1.115~20.161	0.039
脓肿切口（单切口：多切口）	3.246	0.774~5.742	0.042
术后抗生素应用（无：有）	18.346	2.346~19.099	0.429
PRLR表达量（低表达：高表达）	9.099	1.727~14.246	0.016

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